Why I am getting same ct values for three different dilutions?

I am setting up qPCR with human DNA and actin primers, it is working with 10 and 1ng dilution but when I am considering 0.1, 0.01 and 0.001 I a getting same ct values for these 3 dilutions, I am...

08 September 2015 9,674 5 View

Hi, how to get tight pellect from a bacterium which is very slimy?

I am working with a bacterium which is very slimy so I am not able to get the tight pellet, but i need to get tight pellet with very less water for further analysis(pigment analysis)...even i...

01 February 2015 9,703 12 View

This question is deleted by the person who posted it.?

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11 December 2014 6,733 0 View

In gene prediction algorithms are we taking count of enhancers?

I feel the information about enhancers helps us to predict the gene properly, if a gene function is influenced by enhancers then why we r not counting enhancers while predicting gene? some one...

09 October 2014 3,417 6 View

Can any one help me, how do you sequence a virus which contains many modified bases?

Usually bacteriophage contains modified bases, but we don't know what are those modified bases. In above situation how can i go about sequencing such virus.

07 August 2014 8,018 8 View

Can any one please send me original papers on Genetically modified products.Thank you.

I need original papers on Genetically modified products(successful or failed) example:Crops, Food products ,medicinal products. Thank you.

07 August 2014 365 12 View

Hi everyone does anyone have protocols for the following tests?

Acid produced from: Glucose,D-Mannitol,Salicin,Inositol,Sorbitol,Rhamnose,Maltose,D-XYIOX,Trehalose,Cellobiose,Methyl D-glucoside,Erythritol,Melibiose,D-Mannose. Hydrolysis of: Aesculin,...

06 July 2014 6,011 10 View

Is there are any other modified bases in case of T-even Bacteriophages other than Methylation and glucosylation?

Biological DNA modifications have been studied for many years, and much is known about their distribution and the enzymes involved. Well-known base modifications are C-5-methylcytosine (m5C),...

06 July 2014 6,868 1 View

Is it always necessary to memorise everything?

People will always expect that if a person is doing some research they should remember almost all, is it always necessary?

05 June 2014 8,228 2 View

Does anyone have any idea about PFGE (Pulsed field Gel Electrophoresis)?

I do not understand what is happening with the electricity supply (bidirectional), even though the bidirectional bands are very good.. If the electricity supply is bidirectional, then should the...

03 April 2014 1,246 1 View

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02 March 2021 3,060 3 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

What do you know about biotechnology art?

What do you know about biotechnology art?

02 March 2021 1,653 3 View

Which is the best place in the world for the plant biotechnology research?

I am searching for a good place for the Post Doc,

02 March 2021 4,053 3 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Samples to study circular dichroism (chirality)?

I want to build a low-cost polarimeter to study circular dichroism (chirality). What type of samples can I use? I am looking for samples that can be found outside the laboratory.

01 March 2021 9,101 3 View

How to accurately quantify chitin?

Hi all, I have been working on the quantification of chitin in insect samples and feed for a while but I can notice that the quantification methods (e.g. exposure to HCl, NaOH, and weighing sample...

01 March 2021 1,843 2 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Can anyone help with Schistosoma identification?

Can anyone identify what kind of Schistosome species this might be? Morphological features of cercariae and adults resemble Trichobilharzia, though I am fairly new to the field of parasitology...

25 February 2021 7,760 6 View

How was your experience with Biomedical and Biotechnology Journal?

How was your publication experience with BBRJ in terms of peer review and reviewer comments. Did you find it fair and transparent ?

23 February 2021 3,810 6 View