Bhagya C. Thimmappa

22 Questions 58 Answers 0 Followers

Questions related from Bhagya C. Thimmappa

Why I am getting same ct values for three different dilutions?

I am setting up qPCR with human DNA and actin primers, it is working with 10 and 1ng dilution but when I am considering 0.1, 0.01 and 0.001 I a getting same ct values for these 3 dilutions, I am...

09 September 2015 9,781 5 View

Hi, how to get tight pellect from a bacterium which is very slimy?

I am working with a bacterium which is very slimy so I am not able to get the tight pellet, but i need to get tight pellet with very less water for further analysis(pigment analysis)...even i...

02 February 2015 9,801 12 View

This question is deleted by the person who posted it.?

12 December 2014 6,841 0 View

In gene prediction algorithms are we taking count of enhancers?

I feel the information about enhancers helps us to predict the gene properly, if a gene function is influenced by enhancers then why we r not counting enhancers while predicting gene? some one...

10 October 2014 3,543 6 View

Can any one please send me original papers on Genetically modified products.Thank you.

I need original papers on Genetically modified products(successful or failed) example:Crops, Food products ,medicinal products. Thank you.

08 August 2014 455 12 View

Can any one help me, how do you sequence a virus which contains many modified bases?

Usually bacteriophage contains modified bases, but we don't know what are those modified bases. In above situation how can i go about sequencing such virus.

08 August 2014 8,135 8 View

Hi everyone does anyone have protocols for the following tests?

Acid produced from: Glucose,D-Mannitol,Salicin,Inositol,Sorbitol,Rhamnose,Maltose,D-XYIOX,Trehalose,Cellobiose,Methyl D-glucoside,Erythritol,Melibiose,D-Mannose. Hydrolysis of: Aesculin,...

07 July 2014 6,096 10 View

Is there are any other modified bases in case of T-even Bacteriophages other than Methylation and glucosylation?

Biological DNA modifications have been studied for many years, and much is known about their distribution and the enzymes involved. Well-known base modifications are C-5-methylcytosine (m5C),...

07 July 2014 6,958 1 View

Is it always necessary to memorise everything?

People will always expect that if a person is doing some research they should remember almost all, is it always necessary?

06 June 2014 8,329 2 View

Does anyone have experience with how to target a pathogenic bacteria?

By making the pathogenic organisms dns as triple helix (By introducing modified oligonucleotides) so that organism is no more pathogenic.

04 April 2014 1,017 1 View

Does anyone have any idea about PFGE (Pulsed field Gel Electrophoresis)?

I do not understand what is happening with the electricity supply (bidirectional), even though the bidirectional bands are very good.. If the electricity supply is bidirectional, then should the...

04 April 2014 1,347 1 View

Can anybody tell me what changes I can make to a native-PAGE gel with starch to work?

Starch is a polysaccharide. When I provide this as a substrate (before running the gel ) it is not working. Can anybody think of any modification?

03 March 2014 2,932 5 View

Does anyone have a pseudocode for introsort?

I am not able to understand the introsort (when it switches to heap sort and when to quick sort). Please give me the simple pseudocode for introsort.

03 March 2014 1,217 2 View

In what context are defensins produced and do respiratory burst happen?

In phagocytosis there are 2 types of mechanisms: 1) Oxygen dependent: Respiratory burst, 2) Oxygen independent: Through defensins. In what context are defensins produced and do respiratory burst...

02 February 2014 2,527 5 View

Do you have a standardized protocol for plant cell preparation in flow cytometry?

I tried once but I ended up with a noise display. We got a result but it was not a good result. We got good results for mammalian cell prep but not for plant cell prep. We are using Gallios Flow...

02 February 2014 6,884 0 View

We tried to do karyotyping using our own blood sample but we didn't get proper results - any thoughts?

When we did the same thing with HeLa cells we got good results, but not with our own blood sample. I'm not getting what went wrong. I'm expecting chromosome from white blood cells of the blood.

02 February 2014 8,722 4 View

Can anyone tell me about reverse gyrase and hyperthermophiles?

I wanted to know about the lab or institutes where work on above topic is going on and the status of the research.

02 February 2014 5,778 7 View

Can anyone tell me how to arrest all the cells in the metaphase?

I used colchicine but m not able to arrest all the cells in metaphase... I get telophase also... Please can anyone tell me how to arrest all the cells in the metaphase.

01 January 2014 6,850 2 View

What happens to levels of reverse gyrase and DNA gyrase as a function of temperature ?

What is the actual role of reverse gyrase in hyperthermophiles?

01 January 2014 755 2 View

Can anyone tell me the mechanism of action of CTAB on the plant cell wall?

I mean how is it going to precipitate the polysaccharides? Does it precipitate +ve or -vely charged proteins? What about polysaccharides?

01 January 2014 6,788 10 View

What is the main function of the "reverse gyrase" in hyperthermophiles?

Scientists disrupted the reverse gyrase gene from a hyperthermophilic archaeon, Thermococcus kodakaraensis KOD1. An apparent positive supercoiling activity that was observed in the host strain was...

11 November 2013 4,099 0 View

What do methylated bases do in case of tRNA(phe)?

In the case of yeast tRNA (phenyl alanine) there is a G-U base pair (G4-U69), and many bases are methylatedlated like G26(2'methylated), C49(5'methylated), C40(5'methylated), and so on. If we look...

11 November 2013 5,614 2 View