Why I am getting same ct values for three different dilutions?

I am setting up qPCR with human DNA and actin primers, it is working with 10 and 1ng dilution but when I am considering 0.1, 0.01 and 0.001 I a getting same ct values for these 3 dilutions, I am...

08 September 2015 9,674 5 View

Hi, how to get tight pellect from a bacterium which is very slimy?

I am working with a bacterium which is very slimy so I am not able to get the tight pellet, but i need to get tight pellet with very less water for further analysis(pigment analysis)...even i...

01 February 2015 9,703 12 View

This question is deleted by the person who posted it.?

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11 December 2014 6,733 0 View

In gene prediction algorithms are we taking count of enhancers?

I feel the information about enhancers helps us to predict the gene properly, if a gene function is influenced by enhancers then why we r not counting enhancers while predicting gene? some one...

09 October 2014 3,417 6 View

Can any one help me, how do you sequence a virus which contains many modified bases?

Usually bacteriophage contains modified bases, but we don't know what are those modified bases. In above situation how can i go about sequencing such virus.

07 August 2014 8,018 8 View

Can any one please send me original papers on Genetically modified products.Thank you.

I need original papers on Genetically modified products(successful or failed) example:Crops, Food products ,medicinal products. Thank you.

07 August 2014 365 12 View

Hi everyone does anyone have protocols for the following tests?

Acid produced from: Glucose,D-Mannitol,Salicin,Inositol,Sorbitol,Rhamnose,Maltose,D-XYIOX,Trehalose,Cellobiose,Methyl D-glucoside,Erythritol,Melibiose,D-Mannose. Hydrolysis of: Aesculin,...

06 July 2014 6,011 10 View

Is there are any other modified bases in case of T-even Bacteriophages other than Methylation and glucosylation?

Biological DNA modifications have been studied for many years, and much is known about their distribution and the enzymes involved. Well-known base modifications are C-5-methylcytosine (m5C),...

06 July 2014 6,868 1 View

Does anyone have any idea about PFGE (Pulsed field Gel Electrophoresis)?

I do not understand what is happening with the electricity supply (bidirectional), even though the bidirectional bands are very good.. If the electricity supply is bidirectional, then should the...

03 April 2014 1,246 1 View

Does anyone have experience with how to target a pathogenic bacteria?

By making the pathogenic organisms dns as triple helix (By introducing modified oligonucleotides) so that organism is no more pathogenic.

03 April 2014 883 1 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

How to access MSCI KLD Stats and Sustainalytics?

Hello all, I'm currently working on my undergraduate thesis and I'm having difficulties trying to access KLD Stats and Sustainalytics because my campus Airlangga University and apparently other...

02 March 2021 8,525 1 View

Ideas for Phd's Thesis on pricing (Marketing)?

Hi everyone, I'm studying Marketing and I would like to write my PhD thesis on the topic of pricing. Any specific ideas?

02 March 2021 9,706 5 View

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02 March 2021 3,060 3 View

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02 March 2021 932 1 View

How to calculate BREQ 3 questionnaire?

how to calculate BEHAVIOURAL REGULATION IN EXERCISE QUESTIONNAIRE BREQ 3 ,which contain 24 questions and each question has 5 answers (0- Strongly disagree , 1-disagree, 2- Neither agree nor...

02 March 2021 811 3 View

Please suggest a good text book for "Research Methodology in Life Sciences". Which book would be good for PhD Scholars?

Please provide Book Title and author name

02 March 2021 9,059 3 View

How does Scientist/RG community evaluates a case study article and the impact of it on a researcher career?

An article that is published as a case study in the SJR listed journal. How much value is added to a researcher career? How it is evaluated by a scientific researcher/University admission...

02 March 2021 9,661 3 View

Could any one guide me, to write research proposal for grant? ?

We have one plant, the local people using this for controlling blood sugar, the toxicity and antidiabetic activity of the plant is not reported yet, please guide from whag should be the process ?...

02 March 2021 2,300 1 View

How to regulate SSCP temperature in simple PAGE?

I'd like to perform single-strand conformation polymorphism (SSCP) in my thesis, however I cannot control the temperature of the vertical PAGE since we are using the conventional tanks. Is there a...

02 March 2021 9,157 1 View