Why I am getting same ct values for three different dilutions?

I am setting up qPCR with human DNA and actin primers, it is working with 10 and 1ng dilution but when I am considering 0.1, 0.01 and 0.001 I a getting same ct values for these 3 dilutions, I am...

08 September 2015 9,674 5 View

Hi, how to get tight pellect from a bacterium which is very slimy?

I am working with a bacterium which is very slimy so I am not able to get the tight pellet, but i need to get tight pellet with very less water for further analysis(pigment analysis)...even i...

01 February 2015 9,703 12 View

In gene prediction algorithms are we taking count of enhancers?

I feel the information about enhancers helps us to predict the gene properly, if a gene function is influenced by enhancers then why we r not counting enhancers while predicting gene? some one...

09 October 2014 3,417 6 View

Can any one help me, how do you sequence a virus which contains many modified bases?

Usually bacteriophage contains modified bases, but we don't know what are those modified bases. In above situation how can i go about sequencing such virus.

07 August 2014 8,018 8 View

Can any one please send me original papers on Genetically modified products.Thank you.

I need original papers on Genetically modified products(successful or failed) example:Crops, Food products ,medicinal products. Thank you.

07 August 2014 365 12 View

Hi everyone does anyone have protocols for the following tests?

Acid produced from: Glucose,D-Mannitol,Salicin,Inositol,Sorbitol,Rhamnose,Maltose,D-XYIOX,Trehalose,Cellobiose,Methyl D-glucoside,Erythritol,Melibiose,D-Mannose. Hydrolysis of: Aesculin,...

06 July 2014 6,011 10 View

Is there are any other modified bases in case of T-even Bacteriophages other than Methylation and glucosylation?

Biological DNA modifications have been studied for many years, and much is known about their distribution and the enzymes involved. Well-known base modifications are C-5-methylcytosine (m5C),...

06 July 2014 6,868 1 View

Is it always necessary to memorise everything?

People will always expect that if a person is doing some research they should remember almost all, is it always necessary?

05 June 2014 8,228 2 View

Does anyone have any idea about PFGE (Pulsed field Gel Electrophoresis)?

I do not understand what is happening with the electricity supply (bidirectional), even though the bidirectional bands are very good.. If the electricity supply is bidirectional, then should the...

03 April 2014 1,246 1 View

Does anyone have experience with how to target a pathogenic bacteria?

By making the pathogenic organisms dns as triple helix (By introducing modified oligonucleotides) so that organism is no more pathogenic.

03 April 2014 883 1 View

How to access MSCI KLD Stats and Sustainalytics?

Hello all, I'm currently working on my undergraduate thesis and I'm having difficulties trying to access KLD Stats and Sustainalytics because my campus Airlangga University and apparently other...

02 March 2021 8,525 1 View

How to write a scientific report?

We are analysing scientific reports at University in great detail, so I wanted to find a scientific report about how to write a scientific report. Including detail such as what information to put...

02 March 2021 7,602 4 View

How does Scientist/RG community evaluates a case study article and the impact of it on a researcher career?

An article that is published as a case study in the SJR listed journal. How much value is added to a researcher career? How it is evaluated by a scientific researcher/University admission...

02 March 2021 9,661 3 View

Tooth extraction and subsequent dental implant placement in Sprague-Dawley rats induce differential changes in anterior digastric myofibre size and ?

01 March 2021 7,254 3 View

What are the most relevant MSc proposal topics for Petroleum Production Engineering at the moment?

I am a Petroleum Production Engg MSc student at Robert Gordon University in Aberdeen and I am in the process of selecting a topic for my MSc Engineering Investigation proposal. I would very much...

01 March 2021 7,396 4 View

What is the actual chemistry going on between iodate and ascorbic acid?

I am studying biochemistry at university, as part of the course we do several titration experiments. In our handouts, we were given the following half equations: IO3- + 6H+ + 4e- I+ +...

16 February 2021 8,731 1 View

How to accurately measure cytokines levels for my university research proposal?

Hello, i am writing a theoretical research proposal for one of my university assignments regarding Covid-19. The brief is pretty relaxed and i have chosen to conduct a study on how the...

11 February 2021 908 5 View

How many authors?

Hello, I'm currently finalizing my Ph.D. proposal in Research in English Literature. I still have to apply to universities abroad. My question is can I pick two authors for my research (for...

08 February 2021 6,858 12 View

Any good opportunity in foreign universities for Post Doctoral - Spirituality, Teachings of Adi Shankaracharya and Management ?

For pursuing Post Doctoral, from a good university, in Spirituality, Teachings of Adi Shankaracharya and Management, please share relevant information. Thank you.

08 February 2021 5,186 3 View

Does anyone have a copy of the DIBaS dataset?

I am a student from Brunel University London, and I was looking to use the DIBaS dataset for my dissertation. However, recently it has not been accessible via this link:...

26 January 2021 5,185 4 View