I a looking for a witness or compound that is degraded by light and easy to check like color change or something like that.
Just to know is the new amber lights are not causing photo degradation of light sensible compounds.
Generally, action spectra for photodegradation of different compounds have lower values (reduced sensitivity) at longer wavelengths, so amber light would almost certainly be less problematic than white illumination. However, the specific effects on each compound can be different so you may need to check with each of your sensitivie compounds.
http://www.sciencedirect.com/science/article/pii/S0926860X06002973
http://www.sciencedirect.com/science/article/pii/S1011134413001176
Article The photodegradation of some naturally occurring polymers
Thank you for you answer. Basically we want an easy assay to check if the new lights are not emitting UV light. I was thinking about using spiropyrans or something like that changes color under white light but wont change under the amber light.
I will have a look at the links you sent.
Thanks again.
There are two types of amber LEDs: (1) one is the semiconductor amber LED and (2) another one is the phosphor converted amber LED.
Type 1: the semiconductor structure is not good for the range of amber, orange and yellow wavelength. So the color stability and brightness stability are very bad. If you heat your amber LED type 1 from 25°C to 85°C. You can see the color change and brightness change by your eyes. I have no curve of the degradation of the amber LED type 1 here, but I think that, when the stability is bad, the degradation is also not good. You can operate the amber LED type 1 and measure or record by a camera daily, you can see what I have said.
Type 2: the amber pc-LED consist of a bue semiconductor LED in the core and a amber phosphor layer coating it. Although the Stoke-Shift causes the heat problem for the blue chip, blue chip is very stable. Thefore, the stability of amber pc-LEDs is good. I think that for this type it is more difficult to check by a simple method.
Thanks and regards
Vinh
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