Jose Herrera

7 Questions 7 Answers 0 Followers

Questions related from Jose Herrera

Are there different types of Dansyl Chloride?

I was using Sigma Aldrich Dansyl Chloride (03641) suitable for HPLC derivatization on a method for quantification of hormones, but then I changed it for product (39220) for fluorescence and now I...

25 June 2019 1,680 3 View

Do you know ways to remove the ionic supresion?

I am doing some LC ESI MS/MS of Levonorgestrel in human plasma and I am getting a lot of Ionic Supresion. I have tried Liquid-Liquid extraction and SPE but still cant remove it. The column is an...

01 August 2018 4,085 4 View

Loss of internal standard in a LC ESI MS/MS protocol for Gliclazide?

We are performing an LC ESI MS/MS protocol to quantify Gliclazide on plasma samples. We have noticed that the internal standard (Gliclazide D4) has a very high variation compared with gliclazide....

23 July 2018 9,110 4 View

Why in many LC MS/MS techniques the void time is almost the same as the retention time?

I am taking a LC MS/MS course and we are reviewing a lot of literature about different techniques specially in the pharmaceutical industry. The teacher asked us to come up with an explanation on...

16 April 2018 8,765 4 View

We purchased new amber led lights for the lab. Is there a quick easy way to check if they are not causing any photo degradation?

I a looking for a witness or compound that is degraded by light and easy to check like color change or something like that. Just to know is the new amber lights are not causing photo degradation...

10 January 2018 471 3 View

Is there a way to predict the affinity of TLR7/8 binding of a sequence?

I tested different influenza strains in vitro on TLR7 reporter lines and saw differences but want to know if the differences are due to the sequence. I was thinking something similar as with MHC...

07 September 2017 7,314 2 View

Running Influenza virus in protein gels any advice on how to get a clean and nice run?

I have influenza virus produced in eggs and then purified by ultracentrifugation on a sucrose gradient after protein determination to know the amount of virus to put in the protein gel I run the...

19 February 2015 2,467 2 View