We need a validated method to determine 25-OHVit D in human plasma by HPLC-DAD

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Dietrich A Volmer

Hi,

Most labs implement LC-MS/MS methods today but LC-UV (DAD) has been done before. Here are a few examples:

http://www.ncbi.nlm.nih.gov/pubmed/19642101

http://www.ncbi.nlm.nih.gov/pubmed/18969755

William Letter

Try a keyword search on the web (i.e. Vitamin A E D HPLC) to find lots of free examples of UV/VIS methods for these using both NP and RP methods. The web is great resource for this type of information.