Hi all,
I'm trying to image fluorescent stained spleen and I seem to be getting some aberrations on the images, a kind of striping effect. I suspect it is from the laser.
Channels affected are percp and apc. Exposure time 10s.
Platform is Zeiss AXIO with a HXP 120 V.
Has anyone come across this before and/or know of anything that might fix it?
Many thanks either way!
H
The striping effect you're observing on your fluorescence images can be caused by several factors, including laser intensity fluctuations, optical alignment, and detector performance. Here are some potential solutions to consider:
Dear Harry Ward ,
First of all the HXP 120 is not a LASER. It's a wide field white light source. If it is installed on a confocal it is usually used for viewing and finding the right field of view.
Please check if you are really working on a confocal microscope. I would assume form the image you have provided, that your microscope is equipped with an Apotome and that this is in the Apotome mode position while you are acquiring your normal image or wrong calibrated while you are in the optical section (Apotome mode) mode.
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