Processing synovial fluid samples for protease analysis with a mass spectrometer. Samples are centrifuged at 4 degrees 2200g for 15 min, then heat-denatured at 95 degrees for 10 minutes. Samples are then jelly.
Think DNA is causing the issue here. Unsure on how to prevent this from happening.
Tried a centrifuge at room temperature before heat-denaturation but sample was still extremely viscous.