Hello everyone,
I have a problem with my HPLC (e2695), pressure turns to high (300/flow 0.2). i tried wet prime, dry prime, washing with hot water, methanol, 1% acetic acid. and change or check all the wires one by one, even I took out cartridge and column but pressure still is high. Do you have any Idea how can I fix it or is there possible that pressure is because of a trapped-bubble inside? if yes how can I solve it.
Thanks,
I think trap air bubble is not responsible for pressure increase. it is a sort of impurity or particle which trap in line or choke the in line filter this is the main reason according to me. For removal of bubble you simply purge.
Mitra Mazinani: Not an"air bubble". Air bubbles do not obstruct lines. Particles do. You have a clog. Please enlist the help of an experienced liquid chromatographer (Who at your school is in charge of the HPLC instrument? Who provided initial training to the students? Who provides supervision in the use of HPLC system? Get help.). To find the source of the clog, you must be familiar with the entire flow-path of the HPLC system. Is the clog before or after the column? If before, start at the bottle solvent pickup frits and work your way through the system, one section-at-a-time, to find the clog. Start by disconnecting the column (replace with a high pressure union) to see if the high-pressure clog is still present. If the clog is present w/o the column, then leave the column out of the system and begin the troubleshooting process: Make sure the lines are filled with pure, clean solvent for testing (use a single liquid such a pure WATER (if buffers were used), METHANOL or IPA. Set one channel to 100% and try to pump at 1.00 mL/min to waste. Monitor pressure and maintain prime while testing. Follow the lines to the degasser, GPV and pump head, injector, column, detector and check for flow.
Thanks evryone, problem solved! It was due to a blockage in the flow cell and reverse wash fixed it.
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