I would like to make a small volume of emulsion to deliver a vaccine that contains a peptide and DNA oligonucleotide, using PBS for the aqueous phase and Incomplete Freund's adjuvant as the oil. I put 300 ul of each liquid in a 1 ml syringe barrel (plastic) and used a 1/8th inch probe sonicator. I repeat 10 sec bursts alternating with cooling on ice, for several minutes. It forms an emulsion (I'm not sure if it's w/o or o/w, but a droplet of the emulsion fails the dispersal on water surface test. Any suggestions on how to make it more stable? And how do you determine whether it is o/w or w/o? I use such a small volume because the DNA ODN is expensive and we only use 10 ul per mouse.
Thanks
Ed Roy
Hi Edward,
By following the procedure in the above link, you will obtain a stable W/O emulsion
http://www.invivogen.com/PDF/IFA_TDS.pdf
Good luck!
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