Edward J Roy

4 Questions 8 Answers 0 Followers

Questions related from Edward J Roy

For intracellular flow cytometry of IFN in mouse lymphocytes, is it necessary to incubate T cells with peptide for 1 hr before adding Brefeldin A?

What happens if you add the Brefeldin A right after adding the peptide?

09 September 2016 8,055 4 View

Are there IFNg positive cells in non-stimulated splenocytes?

I was setting up a flow cytometry assay for intracellular interferon-g (IFNg), using mouse splenocytes stimulated for 4 hrs with PMA/ionomycin, the last 3 hrs in the presence of Brefeldin A. I was...

03 March 2015 1,092 5 View

Does anyone know why in a bone marrow dendritic cell differentiation stability experiment, after a week will the phenotype still be stable?

We have in the past differentiated C57BL/6 bone marrow cells to dendritic cells using standard techniques (Current Protocols in Immunology), namely GMCSF without IL4. My question is about the best...

07 July 2014 1,669 8 View

Water in oil emulsion in small volume for vaccine

I would like to make a small volume of emulsion to deliver a vaccine that contains a peptide and DNA oligonucleotide, using PBS for the aqueous phase and Incomplete Freund's adjuvant as the oil. I...

01 January 1970 9,961 1 View