I've been trying to perform the warthin-Stary protocol for Leptospirosis in mice kidney.
However, the positivity desapears overnight - Is this normal?
I already tried the oven and the microwave oven...
Hello, everyone. I have tried to determine carrier motilities of some materials, by Density Functional Theory, using Quantum ESPRESSO. There are a few methods to do it, like a package called...
04 August 2024 8,894 1 View
I am tring to culture cells in hypoxia using Cobalt chloride model , but I want to compare with antoher method. Our research is about circRNA expression - qPCR and nanopore oxford sequencing.
05 June 2024 2,246 1 View
Dear colleagues, We are investigating the voltinism of a species of dragonfly in two rivers in Catalonia (northeast of Spain). To study the possible temperature differences between the two streams...
16 February 2024 8,112 9 View
I there meta-analysis of comparative outcomes in unvaccinated re-infected vs vaccinated with 2,3, 4 or more vaccine doses and technologies. ?
08 February 2024 4,322 0 View
Dear all: I would like to perform a post hoc test with R for two independent variables, as well as their interaction. Have someone did it before? I would appreciate any advice. Thanks beforehand
29 January 2024 3,736 6 View
Good day everyone. I'm working with eDNA Metabarcoding for freshwater fishes in Brazil, I ran tests amplifying positive controls and samples with the primer MiFish-U (Miya et al 2015) alone (no...
11 January 2024 5,109 2 View
I have a new publication Ricardo-Suárez F, Lastre-Vera X, Fernández-Domínguez T, Alonso-Montalván M, Curbelo-Valera A. Fascitis necrosante periocular. Revista Cubana de Oftalmología [revista en...
09 January 2024 4,203 2 View
Dear Colleagues, In recent weeks, I have suffered from an issue with the SmartLab SE Diffractometer (Rigaku). Last week, I started the equipment as usual and an error appeared; please see the...
09 January 2024 4,984 10 View
Hello, everyone. I have been using Quantum Espresso to do some calculations, and I noticed something in the output of the process. I use normally QE in Windows10, but I installed Ubuntu via WSL...
13 December 2023 7,934 5 View
Hello, i am doing some research about nanoparticles (NP) and i'm little concerned. Hope i can explain my question. When NP are synthesized i can see the dosage used but i cannot understand how...
18 September 2023 9,621 3 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much
08 August 2024 9,157 4 View
Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...
06 August 2024 9,710 1 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I´ve been unable to find specific information about this neurotrophin in the CNS of rabbits exclusively. There is extensive info in mice, fish and rats, but in brain´s rabbit is hard to find....
04 August 2024 762 1 View
I need to lyse and homogenise mouse tissues. So far I have experimented with liver and lung tissue, using matrix D. The liver was completely lysed with 6 repetitions of the standard program, but...
31 July 2024 9,186 0 View
I need to Postfix mice brains. I slice the brains using microtome at 40 microns. These are Postnatal days 7, 11, 14, and 21. I am using half the brain for other analysis, so I need to fresh freeze...
31 July 2024 1,123 4 View
I am conducting my research regarding blood coagulation in patients with CKD. I observed a reduction in post hemodialysis aPTT and PT values compared to pre hemodialysis values. But most of...
30 July 2024 3,467 0 View
Dear colleagues and scientists: I am asking for your help and insights to develop and explore the feasibility of using an immortalized kidney cell line, for example, HEK293T, for the induction of...
29 July 2024 5,622 3 View