Does anyone indicates a site or a book of "Meta-analysis for dummies"?

24 February 2021 2,755 4 View

Do you think the people can change his activity behavior just because have a chronic disease?

Along several years specialist study the physical activity, bur why not everybody meke changes.

03 December 2020 2,191 3 View

How to reproduce the sub-grade of pavements without using soil?

Dear colleagues, In order to evaluate the performance of concrete slabs-on-grade, the sub-grade should also be considered in the testing setup. Using soil or sand to reproduce the sub-grade is a...

20 November 2020 7,921 1 View

Bioinformatic tools to determine ploidy in microbial genomes?

Looking for tools with proved accuracy to detect ploidy levels in microbial whole-genomes.

14 October 2020 4,273 3 View

How can I determine which dye I'm dealing with?

I am currently analyzing the composition of an unknown solution. It is a blue dye solution, but there are an awful lot of blue dyes. I have already analyzed the UV-VIS spectrum, the wavelength is...

08 October 2020 4,651 4 View

How to protect intellectual property rights in the innovation process?

Not all companies have an R&D area or specialists that supply their need for knowledge and technology, and in this case, they seek solutions external to organizations, through specialists or...

21 September 2020 7,918 30 View

Help with ERROR: Lost atoms in LAMMPS?

Hi, i'm a physics student working in a project of materials science. I'm doing a simulation using LAMMPS. In this case, i have a problem with the error Lost atoms: original 60536 current 56624...

11 June 2020 6,433 4 View

What is the proper statistical test to check if a single time-series is different than a group of multiple time-series of the same variable?

Hello, I have a set of 21 time-series with weekly data of the same variable from years 2000 to 2020 (January to mid-May only). The figure illustrates those 21 time-series. I want to evaluate if...

25 May 2020 863 3 View

Has any one used Deep Learning to forecast fish catch prices?

22 May 2020 4,779 2 View

Can someone recommend papers regarding time course lignin accumulation?

I'm looking for information regarding the dinamic of lignin accumulation during in vitro culture of plants.

16 April 2020 3,699 5 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

IHC on mouse liver hepatocytes?

Can anyone help me choose primary antibodies for mouse liver hepatocytes and also in conjunction Cd8 cells. Also Cd3 cells. I want to look at hepatocytes and adjacent immune cells in mice with...

01 March 2021 6,953 1 View

How to avoid ulcers in s.c. injected tumors ?

Recently, we have injected s.c. B16F10 tumor cells in BL/6 mice. At around 12-15th day some animals showed ulcerations in tumors , with a big hole in the middle (it looks like a volcano). We have...

01 March 2021 9,938 3 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

Looking for genomic DNA extraction kits from mouse tissue (centrifuge-based, not spin-column)?

Hello. I am looking to extract genomic DNA from mouse brain and lungs and I need a kit that is not spin-column based because I need to scale up the solution amounts for 400mg tissue (most cap at...

25 February 2021 155 2 View

What are the pros and cons for mice versus rats?

Hi Everyone, I am designing a pilot study using rodents to assess the effects of simulated space environments (radiation + altered gravity) on microbiome and immune system. I have limited...

24 February 2021 1,825 3 View

Is it harmful to breed for Cre Homozygosity initially?

Hello, I am currently using a Cas9 knock in system that will be expressed in the liver. To do so STOP codon fl/fl Cas9 KI mice were bred with Albumin-Cre mice to generate heterozgous pups, that...

24 February 2021 5,759 3 View

How to empty large intestine in mice ?

Hi all I am working on colon-targeted drug delivery for which I kept mice fasting for 6h using fasting grills during scotophase (dark period). I observed liquid fecal content in the cecum and...

23 February 2021 7,701 2 View

Does PHA-P stimulate the proliferation of lymphocytes from mice?

Hello, I will start performing MLR using lymphocytes from mice's spleen and I would like to know the better way for the stimulation. If is using PHA-P or CD3/CD28? I would like to know as well if...

23 February 2021 5,476 4 View

How to culture mouse pheochromocytoma cells (MPC)?

Hi Everyone, Has anyone here worked with MPC cell line? Any tips or tricks? I am just venturing into cell biology and any advice on this topic would be highly appreciated. Thank you so much. Niusha

22 February 2021 3,891 2 View