Ricardo Camarinho

5 Questions 9 Answers 0 Followers

Questions related from Ricardo Camarinho

Using EDTA to decalcify bone samples for histology how much V/V ?

While using EDTA decalcify bone samples from Mus musculus, how much volume of EDTA is it recomended per piece volume? For the fixation with formaldehyde the normal is arround 20x...

09 October 2019 9,892 3 View

Warthin Stary stain desapearing overnight - is this normal?

I've been trying to perform the warthin-Stary protocol for Leptospirosis in mice kidney. However, the positivity desapears overnight - Is this normal? I already tried the oven and the microwave...

25 October 2018 8,984 0 View

PFA/Glutaraldehyde max time storage for TEM sample preparation?

Hello I'm currently processing brain samples for TEM microscopy. Although the protocol is simple, I'm having a few questions about max storage time between the processing steps. Regarding that...

10 April 2018 1,876 1 View

I'm looking for a full protocol for TNF-alpha immunohistochemistry for parafin sections?

Could anyone name a full protocol for TNF-alpha immunohistochemistry for paraffin sections and antibody reference and brand? Thanks in advance

23 October 2017 2,318 3 View

Peripheral Blood Smear - how to make permanent preparation slides?

HelloI am trying to perform a blood analysis of micronucleus of lymphocytes in mice peripheral blood. However i'm having a little trouble performing the technique to obtain the permanent...

08 November 2016 512 3 View