I am trying to validate DNA Aptamer-cell (Bacterial cell) interaction in vitro with Biotin-streptavidin assay.
- DNA aptamer (41 bp) is labelled with Biotin on 3' end. After incubating aptamer with whole bacterial cell, I am trying to separate aptamer bound cells using Streptavidin coated magnetic beads.
- Then Isolating DNA of the separated cells and gene specific PCR to validate the specific binding of bacterial cells to aptamer.
- But I am facing few hurdles meanwhile. Would love to discuss with expert in the field of Aptamers.
Kindly share and help me connect with expert.
Thank you
Henry Kolge
You can connect with Dr Virendra Gajbhiye from Agharkar Research Institute, Pune
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