Dear Research Scientists

I process of algae, I am positive I have produced Vitamin B12; HPLC analysis shows we have significant amount of our product. The problem is the product is not 100% pure since we have carbohydrates, lipids and fatty acids even proteins all are exist in our sample. In process of purification we know by role of thumb, we need column chromatography for separation of Vitamin B12; while having adsorbent such as Ambelite, Sephadex gel, Agarose, Alginate or even molecular sieves to remove impurities. Our objective is to deliver pure Vitamin B12. Separation and purification must be carried out by suitable solvent. In final stage we should remove solvent and deliver 99% pure product for human use. This means we should use green chemistry solvent; avoid any toxic solvent in extraction process.