VIRUSES - FAQS.TIPS

More Santosh Kumar's questions See All

What is the product size in real Time PCR for Absolute Quantifiction?

I want to know the recommended product size required for best identification and Absolute quantification in Real Time PCR. If any one can give their protocol for both relative and absolute...

31 December 2017 6,995 0 View

I want to perform Quantitative Real Time PCR to detect and estimate Viral load in tissue, how to design experiment for this?

I want perform quantitative Real Time PCR to detect and estimate Viral load in tissue. I have both Control and Experimental Tissue. I have one Target and House keeping primer. Below are my few...

06 July 2016 5,720 5 View

LIFE OR NO LIFE

HOW TO DEFINE VIRUS?

01 February 2011 3,883 7 View

hi nevidita

wht is PGPMs?

09 October 2010 817 1 View

Hi, I am Working on Somatic Embryogenesis

Can anyone help me in Somatic embryogenesis, i mean in paper.

08 September 2010 2,771 0 View

What is the composition of cryopreservation medium for virus in -80?

Hello all. I grew ARPE-19 cells in cell culture and infected them with a virus (Varicella-zoster virus). After the virus reached a high infection rate, I harvested everything in the plate and use...

07 May 2024 259 3 View

How freeze/thaw cycles affect virus culture fluid titers?

Hi follow virologists, I'm new to the virology field and I'm eager to understand how freeze/thaw cycles affect virus culture fluid titers. I've noticed that while some viruses show increased...

18 March 2024 5,174 4 View

What is the PCR technique used in Virology for the detection of viral nucleic acids for RNA and DNA viruses?

Dear virologists; What is the PCR technique used in virology to detect viral nucleic acids? The steps involved. I'd also like to know, since some viruses have a single strand of DNA and RNA, how...

28 February 2024 1,869 10 View

May viral DNAs recombine with non-homologous recombination in eukaryotic cells (e.g., with non-homologous end joining)?

Is it possible that a hybrid virus is generated in a eukaryotic cell by co-infection of different viruses through nonhomologous recombination?

14 September 2023 7,245 3 View

RT step- Can I use 2 different target reverse primers instead of random hexamers? For 2 different PCR reactions on the same "first strand" RT product?

For diagnostics of Avian infectious bronchitis viruses, I use an end-point RT PCR reaction, and before the RT srage I do a primer annealing stage with the target reverse primer (2uM). My queation...

22 August 2023 1,600 1 View

Do you research project? Are you looking for a competitive candidate for your project?

I am looking for a PhD Position in Microbiology, Virology, and Molecular Biology.

26 July 2023 7,550 1 View

I am seeking of NZ2 strain ORFV virus. Is there the virus in your labs collection?

Dear Researchers, I need 2 ml of NZ2 strain ORFV virus for my student Ph thesis. Could you send us, buyer pay. My address: Prof.Dr. Mehmet KALE Department of Virology Faculty of Veterinary...

29 May 2023 7,105 6 View

Why HeraLabs supply piggybac transposase as a non autonomously replicating plasmid?

Hi, I have been reading about vectors and strategies used in engineering insect cell lines. PiggyBac being a very promising system, is not supplied with a autonomously replicating transposase...

17 April 2023 5,873 6 View

Where to purchase viruses other than ATCC?

I have been looking to expand our virology department here for a year since joining my current institution. I have only ever purchased viruses from Carolina Biosciences (bacteriophage) and ATCC...

12 January 2023 4,248 2 View

What are the universal primer sequnces for H5N1 amplification by RT-PCR (for phylogenetic analysis purpose)?

Im trying to find an oligoprimer set for HA gene amplification of H5N1? I will appreciate if you could point out a primer set for phylohenetic analysis by H5N1 HA gene sequencing.

27 November 2022 9,614 2 View

Ashok Mishra

35 S promoter from Cauliflower mosaic virus is already being used in transformation protocols.

Nuclear polyhedrosis virus (NPV) is used as a biocontrol agent to kill insect pests in cotton and other crops.

Asim Shaghil

Hi, Santosh You can use viruses and scientist are using it in many areas whether it is agriculture, Molecular Medicine or Etc. But FDA regulations and Involment of Life health hazards are a bit of big hassel.

sudhir kumar Sinha

viruses has so many uses as well .

eg: 1.Antobody Production Against plant/Animal/Human Diseases

2.Plant/Animal/virus based Recombinent Protein Production

3.Biopesticides Development.

4.Disease Resistant plant Development

5. Biowepons Development