We plan to do co-staining for smooth-muscle-actin and a fibroblast marker in paraffin-embedded lung tissue. For SMA we routinely use the mouse 1a4 antibody. In order to distinguish fibroblasts from other cell types (e.g. VSMCs), we would need another fibroblast marker antibody (e.g. vimentin) which is not produced in mouse, but preferably in rabbit or goat. Has anyone experience with an antibody and could recommend it?
Thanks a lot!
Hi Clara,
www.sysy.com rb vimentin No: 172002 or
www.abcam.com rb mab vimentin ab92547, here you can recieve a testing aliquot (10µl) for 99,- EUR. You can also aske Synaptic Systems (SySy) for a testing aliquot.
Good luck!
Zytomed systems Cat. No. 522-3204 they are quite cheap and reliable. You can get also testing aliquot of 50ul for around 50 Eur.
Best,
Ivan
Dear all,
Thanks a lot for your suggestions. We now ordered the ab92547 and will give it a try.
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