Dear Research Gate scientists
I have both Vero and HepG2 cell lines growing in flasks using high-glucose DMEM media. Can I switch to low-glucose DMEM for the next passage, or should the media be changed gradually?
I am currently investigating the CRISPR 9 case to target my gene of interest for knockout. The vector being used is lentiCRISPR v2puro. I digested and dephosphorylated 5ug of the lentiviral...
03 June 2024 2,877 7 View
Good morning Research Gate scientists I have an LB agar plate for DH5a bacteria cell storage at 4C for 6 weeks, and I tried to subculture to a new plate plenty of times but I could not get...
03 April 2024 8,334 3 View
I am currently working on a CRISPR Cas9 project that involves preparing DH5a competent cells for bacterial transformation. I conducted heat-shock transformations using PUC19 as a positive control...
09 March 2024 8,992 1 View
I'm currently in a project involving CRISPR Cas9 for gene knockout and have initiated gene cloning following the protocol from the Zhang lab. My next steps include performing a ligation reaction...
28 February 2024 1,556 3 View
I am going to prepare competent Escherichia coli for use in transformation. Usually, when I conduct a starter culture and overnight culture, I add ampicillin. However, in the protocol, they did...
05 February 2024 7,886 2 View
I am going to culture the HepG2 cell line for the first time, and I have some questions If someone could help with these questions, I would appreciate the help When we revive the cells with the...
23 December 2023 8,915 5 View
Hi all, Anyone can help me to fix the problem, VERY BAD NEWS! internal error in subroutine IBZKPT: Reciprocal lattice and k-lattice belong to different class of lattices. Often results are still...
20 August 2023 8,107 2 View
My Calculation run in VASP, and the calculation has a problem. So, how I can solve this problem, please?
10 April 2023 5,914 7 View
There are two methods of education. They: (1) Teacher-centred learning (2) Student-centred learning what do you think? What better way? And why?
14 July 2019 10,058 10 View
Hello, is it possible to design odd number of patch antennas with corpotate feed network or its possible just with pair number of antenna? help please.
05 August 2018 6,978 1 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
Hello, everyone. I have tried to determine carrier motilities of some materials, by Density Functional Theory, using Quantum ESPRESSO. There are a few methods to do it, like a package called...
04 August 2024 8,894 1 View
Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...
04 August 2024 7,282 2 View
I have used PE and PE-Dazzle 594 fluorochromes and have managed to get NK cells to properly show GranzymeB expression after 4 hr PMA/ionomycin stimulaton, but for some reason my CD8 cells in the...
01 August 2024 7,677 2 View
What types of mask designs for metal deposition, to be used in a PVD system, are best suited for perovskite-based solar cells?
31 July 2024 4,835 3 View
Hi there! I recently started working on Lx-2 cells. When I thaw the cells, they start attaching within few hours but later in about 2 days they started rounding and dying. I used DMEM+2% FBS...
31 July 2024 3,893 0 View
Hi all, may I ask you for advice about treating monolayer culture cell line with essential oil. Due to the hydrophobic character of the oil, I am concerning how can it equally affect to all the...
31 July 2024 5,301 2 View
As animals learn a task, they become more reliant on their long-term memories as compared to the real-time sensory information to guide behavioral performance (Ahilan et al. 2018). This process...
31 July 2024 9,859 0 View