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I am currently investigating the CRISPR 9 case to target my gene of interest for knockout. The vector being used is lentiCRISPR v2puro. I digested and dephosphorylated 5ug of the lentiviral...
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Good morning Research Gate scientists I have an LB agar plate for DH5a bacteria cell storage at 4C for 6 weeks, and I tried to subculture to a new plate plenty of times but I could not get...
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Dear Research Gate scientists I have both Vero and HepG2 cell lines growing in flasks using high-glucose DMEM media. Can I switch to low-glucose DMEM for the next passage, or should the media be...
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