Validation of NGS taxa abundance data? or any other method (experimental or bioinoformatic based) to validate NGS results?

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Sandipan Samaddar

Hi,

Can you please elaborate what you would like to validate? Like abundance data of particular taxons or some functional gene responses?

Thanks,

Sandipan

Rakeshkumar Yadav

Abundance data of few taxa.

I agree with Thomas and also you can carry out RT_PCR as you pointed out using specific primers targeting the particular taxon. If I come across any articles in the mean time I will share with you definitely.

Thanks.

Thanks for your responses. But what if it is a community metagenomic sequencing and not the targeted amplicon sequencing? Can we still include mock samples? Please provide an elaborate explanation. Will be grateful for your time. Thank you.