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My goal is to align my metagenomic sequences with virulence gene database in order to find out which probable genes are prevalent in the data. So I ran diamond aligner using following...
10 November 2019 2,486 2 View
I am analyzing a meta genomic data and I want to comment about the significance of beta diversity for the samples taken from different locations. In order to do that i did Adonis test (seed of...
07 August 2019 4,103 4 View
I am looking to validate the information generated from metagenomic sequencing. I know that I can carry out qPCR to validate my data. And I guess Random forest regression analysis can be done too....
03 April 2019 7,274 1 View
Previously, I have done illumina data analysis so have experience in doing that. However, Nanopore is bit new to me and I am quite confused in its data analysis. I have epi2me (I guess it uses...
09 October 2018 7,816 0 View
We all know about the specificity of viruses or phages and I guess that would specially limit their enrichment to certain groups. Suppose I have a sediment or sewage sample and I want to enrich...
08 September 2018 6,448 15 View
I am trying to understand mantel test in terms of getting to a biological conclusion. I referred few papers but failed to understand it completely. I have understood that it is a correlation...
08 September 2018 5,451 1 View
Hello, I have been looking out for studies which have actually tried to validate the results from NGS data. One technique I know is Real Time-PCR. I was actually looking out for real time PCR...
08 September 2018 3,346 4 View
I have been trying to extract DNA with no success. I have used following methods 1. PCI method with and without bead beating 2. kit methods qiagen, himedia 3. interchange of reagents 4. cold and...
11 December 2017 7,943 4 View
source organism is also known. nearest match for structure with 80% similarity is found. is it possible?
11 December 2016 6,991 3 View
Recently, I read an article on AMF succession. In order to explain the dissimilarity in AMF Counts, they have used PCoA plot. Mostly , I have seen the PCoA plot are being constructed by using...
01 January 1970 4,554 0 View
We intend to study the interaction between peptides and polymer (like PP, PE and PS) through MD simulations using Martini force fields ( Martini 2 for PP and Martini 3 for PE, PS). We have...
08 August 2024 4,842 0 View
The paper in question is "Interpolation of Nitrogen Fertilizer Use in Canada from Fertilizer Use Surveys". This paper was very recently published by Agronomy (MDPI). Agronomy has, in the last day...
07 August 2024 9,934 3 View
I am not able to get good literature and the physics behind how first these grains and grain boundaries arises out of no where when we make a pellet to study its dielectric properties and then how...
07 August 2024 5,177 3 View
Why is the molecule's orientation with an electric field affect polarizability? Electrons are diffuse enough to be independent with respect to orientation and effect of electric field on...
03 August 2024 7,843 1 View
If you think of electrons with spin as bar magnets, you know bar magnets of opposite polarity as long as they're not occupying the same spatial location don't cancel out each other's magnetic...
03 August 2024 3,927 2 View
I am using a Bruker 600M solid-state NMR spectrometer with a Micro 2.5 microimaging system. The test sample is a tube of 1M LiCl aqueous solution, and the nucleus detected is 1H. I am trying to...
01 August 2024 9,227 1 View
Following curve related to Co0.6Mn0.4Fe2O4 Magnetic nanoparticles with extra phase a-Fe2O3. I will appreciate to help me to interpretation this spectroscopy, especially the blue line.
31 July 2024 9,140 2 View
I want to know more about ore deposits in world.
27 July 2024 3,827 4 View
Does it exist some special technique for cutting ceramic membranes for Cross-sectional FESEM or SEM images?
25 July 2024 5,482 2 View
We assume that there is a physico-statistical meaning to the constant π other than circular geometry, but the iron guardians of the Schrödinger equation deny this. The iron guardians of the...
22 July 2024 4,285 9 View
