How to model the battery pack in PSIM or PSCAD?

Hello, I have simulated a single battery cell using Thevenin equivalent circuit. For a battery pack, I have a large number of cells connected in series and parallel connections. Is there any way...

28 February 2021 2,116 1 View

How to prevent ANFIS Model from negative prediction values in MATLAB?

I have input and output data set for "ANFIS modeling in MATLAB", and I am getting some negative predicted values of output in testing. However, the predicted values of output in training are...

28 February 2021 3,459 3 View

Why according to FRAC (fungicide resistance action committee) phenylamide fungicides application interval should not exceed 14 days?

Why according to FRAC (fungicide resistance action committee) phenylamide fungicides application interval should not exceed 14 days. Anyone know reason behind this strategy

18 January 2021 1,166 3 View

What are the political factors affecting curriculum design at the primary level in Pakistan?

This Topic I chose for my Thesis in Final Year of my Master's. I am in process of it's completion but need more suggestions and comments for the Topic to elaborate.

14 December 2020 356 3 View

How to calculate the replacement cost of battery?

As battery needs to be replaced after the specific charge and discharge cycles mentioned in the datasheet. If we have the time series data of battery power and energy capacity, then how can we...

01 December 2020 3,433 3 View

How to coat oleic acid on fe3o4 nanoparticles and yo confirm visually?

I made iron oxide nanoparticles via co-precipitation method and obtained a brown colour slurry with neutral pH and I did ex situ coating of oleic acid on nanoparticles and washed it with ethanol...

10 November 2020 5,911 4 View

Can anyone please elaborate the relationship between charge and discharge cycles and battery lifetime?

I am designing the optimal HESS capacity for the renewable system. I have to select the cutoff frequency of low pass filter to divide the low and high-frequency components. charge and discharge...

05 November 2020 8,397 3 View

How to select the frequency range in MATLAB Wavelet toolbox?

I have a continuous, time-domain noisy signal and want to decompose into low-frequency (LF) and high-frequency(HF) components using Wavelet Toolbox. But, I am unable to select the frequency range....

29 October 2020 5,219 5 View

How to confirm coating of oleic acid without using any XRD,SEM,FTIR???

I made iron oxide nanoparticles via co-precipitation method and obtained a brown colour slurry with neutral pH and I did ex situ coating of oleic acid on nanoparticles and washed it with ethanol...

28 October 2020 1,802 3 View

Hello everyone, I have GCMS and IR spectra and bioactivity results of essential oil. Can anyone tell me the the computional software for further work?

Hello everyone, I have GCMS and IR spectra and bioactivity results of essential oil. Can anyone tell me the the computional software for further work?

16 August 2020 6,197 3 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

Buffer to disrupt platelet alpha granules for PF4 quantification?

Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...

03 March 2021 1,499 2 View

Buffer Exchange using column packed with sephadex G-25 effect on protein concentration?

Hello, If i am doing a buffer exchange for an antibody of 1mg/mL, does the elution lose protein in the process of buffer exchange? For example, if i flow through 500 uL of 1mg/mL sample, and...

03 March 2021 6,299 3 View

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02 March 2021 932 1 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

Why are my bands weird in this Agarose gel?

Can someone please give me some possible things that could go wrong? Here is my recipe: 0.5g Agarose 50 mL of TAE 1x 1 uL ethyl bromide. Gel was run at 100V for 1 hour. The buffer used is also TAE.

01 March 2021 9,952 3 View

What do I do with DLS peaks that are from the buffer?

I'm looking at the aggregation of my protein sample using DLS. Unfortunately, my buffer (20mM HEPES) also results in a set of peaks. These are at approximately 1 and 1000 d.nm. The lowest peak...

01 March 2021 9,015 2 View

What is the most suitable resuspension buffer for TCA/acetone precipitated protein?

I need to extract protein from fermented carbon sources for Bradford assay. Most researchers experiencing insolubility of pellet in resuspension buffer. Please assist me to select most suitable...

26 February 2021 7,129 3 View

What urea buffer can i use for biotagged protein extraction from streptavidin beads?

Hi all, I have been doing research on biotagged LDB1 proteins in Mel cells. I purify the proteins using M280 streptavidin beads. The yield is very low so I've been trying to optimize it by...

24 February 2021 5,749 3 View

Cloudy/high background western blot - can anyone help me?

Hi everyone! I try to detect VDR protein (~48 kDa) using Western Blot. After SDS-PAGE using 10% MP TGX Stain-Free Gel from BioRad I transferred the protein onto an metOH activated PVDF membrane...

23 February 2021 6,266 3 View