Anyone with some Transgenomic Wavemaker software? Seeking help from the chromatography community?

I am trying to seek out some Transgenomic Wavemaker software (happy to purchase a computer too) to operate a fraction collector. The only way to connect to the fraction collector so far is to...

23 February 2021 9,181 1 View

Best way to optimize species-specific primers? Can I use a temp gradient to test a Ta where I will get target (and no non-target) amplification?

Hello, I am testing different species-specific primer sets. I have two targets (two species, but same gene target). I am trying to select the best primers -- one that will amplify the target...

18 January 2021 1,981 6 View

Summer Studentship opportunities in Analytical Chemistry LC-MS HPLC Chromatography?

The ChromSoc/BMSS 2021 Summer Studentship Applications are now OPEN (DEADLINE 15th JAN). ChromSoc & BMSS , annually funds research projects to take place over the summer . The scheme provides...

17 December 2020 5,535 3 View

How do make a horizontal Boxplot incl. Outliers? (Excel, SPSS, others)?

I have recorded a time series (in minutes + seconds) and would like to make a horizontal boxplot. I have vertical boxplots in SPSS and Excel. However, Excel is not able to make horizontal boxplots...

30 November 2020 9,005 6 View

Has anyone generated Flp-In host cell lines from HeLa TRex cells? If so, anyone willing to share a detailed protocol ?

I am trying since 2 months to generate HeLa Flp-In TRex cell lines, but failed. If someone have a detailed protocol, including cell number to seed, transfection method used, antibiotic...

22 November 2020 5,723 3 View

Statistics on prevalence of methodological approaches in psychological research?

I am currently looking for data on psychological research. I am interested in -current and previous- prevalence of methodological approaches in psychological research (e. g. the proportion of...

15 November 2020 5,711 3 View

Does Formic Acid linger in the MS?

Does anyone know if formic acid has a tendency to linger in the MS after organic solvent cleaning? If it does, are there any special processes that can be put in place to remove it? Thanks!

11 October 2020 3,828 2 View

SPSS frequencies bar chart?

Hello, is it possible to get a bar chart in SPSS where I have the frequencies on the y-axis and two variables on the x-axis? How do I get the two variables on the x-xis? thank you

01 October 2020 6,876 7 View

What are the effects of Covid 19 on nursing students and faculties?

Due to crisis of Covid 19 students have to disconnect from their education system and for preparing nurses its must to relate the theory with skill labs and then hands on practice is required to...

17 May 2020 7,462 21 View

How do I compute for the Pr(Y=1|X=1) H1 and Pr(Y=1|X1) H0 for a priori estimation of required sample size?

I am trying to estimate the required sample size replicate a study which used a probit regression analysis. The paper reported the probit regression results and the marginal effects but I can't...

17 May 2020 4,826 1 View

Waters 2707 HPLC autosampler problem?

I am using a 2707 waters HPLC device. When I try to inject a sample, it says missing plate or rack. I changed the needle and calibrated its position but I still get the same problem. I even get...

02 March 2021 1,408 1 View

Should we change media after1st 6hrs after plating HUVEC bought from INVITROGEN?it's not recomended by invitrogen.Will DMSO present in stock matter?

We have HUVEC bought from Invitrogen. Cat no -C-003-5c. while first plating the cells we have bought the protocol suggests not to centrifuge the cell with media to remove the DMSO. Should we...

02 March 2021 3,713 2 View

How to normalise data on OPM package from R Studio ?

Good afternoon, I recently used OmniLog from BIOLOG for my experimentations : I tested the metabolism of different strains on 2 types of plates. I have 16 strains of 3 different groups...

02 March 2021 3,584 1 View

Benefits of the 24 welled plate?

We have always used the 96 welled plate, the 48 welled plate but apparently there is much evidence on the 24 welled plate and above all its benefits.It states it is the same you would use on ELISA...

28 February 2021 1,149 1 View

Can bacterial suspension be pipetted directly onto petri dish and agar poured onto it?

Hi, recently I encountered a protocol for plating which was not the usual method I have been practicing (pour LB agar onto plate, allow it to solidify, pipette bacterial suspension onto plate,...

28 February 2021 7,292 3 View

What is the best surface to culture cells isolated from a skin biopsy?

Hello, recently I am having some difficulties with maintaining the cell culture of primary cells isolated from a skin biopsy (normal and malignant). The coating of culture plates with Matrigel (a...

26 February 2021 6,151 2 View

Difficulty in cloning of 1kb gene into vector?

I am facing difficulties in cloning a 1kb gene into a vector (pJIT163). I have my gene on interest (GOI) in pUC57 and want to clone in pJIT163 using SalI and BamHI restriction sites. I am getting...

25 February 2021 3,221 7 View

Can cells be reused after using WST 8/ CCK 8 from Sigma Aldrich?

Hi all, Can cells be reused after I have seed them onto 96 well plates and test them with cell counting kit at OD450 (CCK8) at 12 hours, wash them with PBS x3 and add new media and let them...

24 February 2021 5,072 1 View

How to handle studies which report perfect effect sizes in a meta-analysis using Pearson's r?

I'm in the process of updating a meta-analysis and in one of the new studies, effect sizes are reported as Tau-U. Two of the effect sizes are reported as Tau-U = 1.00. I am using Andy Field's...

24 February 2021 2,265 3 View

Standard curve shifting over time. How do I stabilise the ELISA?

24 February 2021 1,365 3 View