• HI all I am trying to follow this recipe, but I do not have Na2HPO4-7H20, can I substitute this reagent with something else and how would that change my ratio?

Recipe for Buffer 1: 10 mM PBS, pH 7.4

Note: These recipes are designed to make the common buffers mentioned in our procedures. This list is not all inclusive. Use NaOH or HCl to adjust pH, being careful not to overshoot and back-titrate, as this may alter salt concentration more than necessary.

Combine:

• 0.26 g KH2PO4
• 2.17 g Na2HPO4-7H20
• 8.71 g NaCl
• 800 ml dH20

Adjust pH to 7.4 and bring volume to 1 L with dH20