Greetings to all of you,I am studying the urea denaturation of an intrinsically disorder protein using urea range from 0.1M 0.2M etc upto 5M.With 0.1M emission intensity is decreasing and then with increasing urea 0.2M the emission intensity is increasing.Then further with increasing urea concentration the emission intensity is decreasing and increasing upto 3M.Beyond 3M the signal intensity of emission reaches to zero.How will I speculate this studies.Any help Please