Abrar Hussain Qadri

4 Questions 9 Answers 0 Followers

Questions related from Abrar Hussain Qadri

Is it possible to obtain the cryo-EM structure of a purified protein that is GST-tagged and without cleaving the tag?

Greetings to all. Currently, I have successfully purified the GST-tagged fusion protein. The protein degrades as soon as I remove the tag. It is an inherently disordered membrane protein. Please...

26 September 2023 6,834 1 View

Urea Denaturation studies of a intrinsically disordered protein?

Greetings to all of you,I am studying the urea denaturation of an intrinsically disorder protein using urea range from 0.1M 0.2M etc upto 5M.With 0.1M emission intensity is decreasing and then...

29 October 2022 9,862 0 View

How to speculate the Protein oligomerisation?

Greetings everyone! I'm trying to find out one of the protein's oligomeric nature. I used three different approaches to identify the oligomeric status. I found the range of oligomerization, from...

08 October 2022 4,985 5 View

SPR PROTOCOL surface plasmon resonance.?

I Have purified two proteins both are having the HIS tag and now i am planning to check the protein protein interactions by surface plasmon resonance. could anyone please help me how can i start...

29 August 2022 908 1 View