I want to use LNCaP for some molecular studies.So,I just wanted to know the highest passage number of this cell line which can be used for these kind of studies
Very difficult to answer. In simpler terms, its advisable to cryopreserve cells very early in their passage and use early passage cells (probably less than 20-30 passages) to avoid genetic or epigenetic changes, which cancer cells frequently undergo due to dysregulation in growth control. I doubt any literature exists related to your question.
Normally, if you have tumor cell lines from collections, they have to be cultivated properly without any selection. Special rules about cell passages we use usually for primary cells in culture, so just be careful and look for the morphology of the cells to keep the same type as at the beginning.
after passage 35 aprox. the cells change their morphology and become neuroendocrine and get a hormone resistant phenotype
I wouldn't use cell lines that have been passaged longer than 15, but this is personal preference. I know that 20 is common in our institute.
Dear lalita,
Kindly refer to the link that I am posting in this box.Hope that will Solve all your Query regarding Passage Number.
http://www.atcc.org/~/media/PDFs/Technical%20Bulletins/tb07.ashx
I havent worked with this cell line but with other cancer cells, we usually dont go beyond passage 18. But I have a serious question for all the people who have commented. Are the cells actually in passage number 10 or 12 or 15 what you say.. Like some one said earlier that culturing the new cells and freezing them and using the freezed cells again .. But are these cells also in actual passage range.
Actually, passage number is actual in cell collections (depositories). We hope, that all people are correct with passage counting, but sometimes it is not. Anyway, it is very important for normal cells and primary cultures of normal cells. Most of cancer cells practically have no limits of passages.
Go for low passage number as low as possible let say upto 15-20. As it has been shown by Peter Jones group that longterm culturing already induced quite some chages in the cancer cell lines. But people still uses these cell lines upto 100 passage number and keep publishing the results. However, don't forget to perform STR profiling for authenticity of cell line before you start experiment and within 6 months after fishing experiments. Since most of cancer research journals request that before final submission of article.
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