Is there anyone working with uPAR KO mice who could help me by providing two females and one male to grow my colony again? Of course, we will cover all expenses.
Prior to selection three days after transfection I transferred the cells from a six well to a 10 cm plate. One day later I admitted selection medium (DMEM + glutamin, 10% FCS and 10 ug/ml...
02 March 2016 4,552 13 View
I read papers where they dissolve lipids in Ethanol or in Ethanol/DMSO (1:1, v/v). Is it also possible to store glycosphingolipids in DMSO alone to avoid possible ethanol evaporation?
11 December 2014 7,599 10 View
I want to perform co-localization studies. My secondary antibody anti-rabbit Cy2 has a very high background. Actually, there is no difference between only secondary antibody negative control and...
31 December 2013 2,397 2 View
In my opinion it is not feasible to use mouse anti-human antibodies for mouse cells or mouse tissue. But I am not an expert in immunology and I saw publications where people used mouse anti-human...
04 May 2013 1,728 1 View
How do people usually check if mTORC1 is activated? By checking if S6K1 is phosphorylated? How does mTORC1 get activated? Also by phosphorylation or is it transcriptionally regulated?
11 December 2012 8,252 3 View
Does anybody know anything which can be done to get them back to breeding? Are they too old or could there be another reason as to why they are not breeding?
10 November 2012 8,672 10 View
We have major problems detecting sr-b1 in Huh7 cells (liver cells) using the antibody from NB400-104 from Novus Biologicals. Does anybody have experience with this antibody concerning lysis of the...
03 April 2012 6,800 7 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much
08 August 2024 9,157 4 View
Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...
06 August 2024 9,710 1 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
I am working on Abaqus/Explicit(Quasistatic ) for the deformation of the auxetic structure model. Please explain how the plastic input value should be considered from the true stress-strain curve...
05 August 2024 454 3 View
A Markov-like Model for Patient Progression" Markov Chain Monte Carlo (MCMC) Markov Chain Monte Carlo (MCMC) is a powerful computational technique used to draw samples from a probability...
05 August 2024 10,079 0 View
Hi everyone, If you have written or come across any papers where Generalised Linear Mixed Models are used to examine intervention (e.g., in mental health) efficacy, could you please share the...
04 August 2024 4,130 4 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I´ve been unable to find specific information about this neurotrophin in the CNS of rabbits exclusively. There is extensive info in mice, fish and rats, but in brain´s rabbit is hard to find....
04 August 2024 762 1 View
I am working on a SEM model where i have 7 latent variables (6 exogenous and 1 endogenous). In AMOS when I co-vary the exogenous constructs, only 2 paths are coming significant out of 6. But when...
03 August 2024 6,028 4 View