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Questions related from Nicole Paland
Prior to selection three days after transfection I transferred the cells from a six well to a 10 cm plate. One day later I admitted selection medium (DMEM + glutamin, 10% FCS and 10 ug/ml...
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I read papers where they dissolve lipids in Ethanol or in Ethanol/DMSO (1:1, v/v). Is it also possible to store glycosphingolipids in DMSO alone to avoid possible ethanol evaporation?
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I want to perform co-localization studies. My secondary antibody anti-rabbit Cy2 has a very high background. Actually, there is no difference between only secondary antibody negative control and...
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In my opinion it is not feasible to use mouse anti-human antibodies for mouse cells or mouse tissue. But I am not an expert in immunology and I saw publications where people used mouse anti-human...
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Is there anyone working with uPAR KO mice who could help me by providing two females and one male to grow my colony again? Of course, we will cover all expenses.
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How do people usually check if mTORC1 is activated? By checking if S6K1 is phosphorylated? How does mTORC1 get activated? Also by phosphorylation or is it transcriptionally regulated?
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Does anybody know anything which can be done to get them back to breeding? Are they too old or could there be another reason as to why they are not breeding?
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We have major problems detecting sr-b1 in Huh7 cells (liver cells) using the antibody from NB400-104 from Novus Biologicals. Does anybody have experience with this antibody concerning lysis of the...
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