We want to evaluate mitochondrial mass. Cells were stained with MitoTracker Green FM (Cat. M7514), whose fluorescence monitors total mitochondrial mass and in particular, we want to study mitochondrial mass at basal conditions and after the treatment with CCCP. Unexpectedly, we found that cells treated with CCCP at different concentrations, had a higher MTG signal.
We have repeated the experiments several times and we always get an increase in the MTG signal. We have used different concentrations and time.
How can I explain such results?
We would be very grateful if someone could help us to solve this question
Thank in advance
I'm afraid I don't have an answer but I saw the same thing with Mitotracker Deep Red (MTDR) with 10 uM CCCP and MTDR at 25nM.
I was working on a human bronchial epithelial cell line (BEAS-2b)
The shift was about the same as when CCCP and chloroquine were used together
Check this paper:
PMID: 27065339
doi: 10.3389/fncel.2016.00076
"Flow Cytometry-Based Assessment of Mitophagy Using MitoTracker" by Xiao et al. They have shown that Mitotracker green accumulation is enhanced upon CCCP treatment.
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