I want to know that, when a heterologous gene is expressed under CMV promoter in mammalian cells, what is the percentage of this heterologous gene's mRNA in comparison to total cellular RNA and total cellular mRNA? Is there any mention of this in the literature?
The expression of a heterologous gene under a CMV promoter will vary depending on the particular gene, its sequence, and the target cell type. Generally, the expression of a heterologous gene under a CMV promoter is stronger than its expression under its own native promoter. The amount of mRNA produced by a heterologous gene compared to total RNA and total mRNA in a cell will also vary depending on the particular gene and its expression level.
Any transient transfection can be titrated to achieve a desired expression level. The CMV promoter is very strong and will work in most cell types. The mRNA and protein levels for your gene will vary depending on RNA stability, size, translational efficiency, etc. So, by transfecting varying amounts of your expression vector mixed with something inert, like Salmon sperm DNA, you can empirically determine how much you wish to express. For 100mm plate we use 6ug DNA with lipofectamine or fugene. But that can be 100 ng of vector with 5.9 ug inert DNA. Vector can be anywhere from 100ng to 6 ug.
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