Mohd Ahmad

4 Questions 11 Answers 0 Followers

Questions related from Mohd Ahmad

Is there any hightroughput method to measure the protein expression levels of 100's of protein variants simultaneously when transfected in human cell?

Hi, I have a synonymous variant library of a protein, and it has hundreds of variants. They are cloned in the Flp-In T-REx expression vector to work with the Flp-In system. We have worked with...

23 July 2023 3,955 1 View

Under CMV promoter, how strongly a heterologous gene is expressed? What is heterologous gene's mRNA % compare to total RNA & total mRNA in a cell?

I want to know that, when a heterologous gene is expressed under CMV promoter in mammalian cells, what is the percentage of this heterologous gene's mRNA in comparison to total cellular RNA and...

19 April 2023 5,954 2 View

Any optimized protocol for nucleofection of EBV-transformed lymphoblastoid B-cell lines (LCLs) with CRISPR vectors (12kb)?

I am trying to knock down a gene in EBV-transformed lymphoblastoid B-cell lines (LCLs) using the CRISPR system. For this purpose, first I tried the lipofectamine mediated transfection of cells but...

13 October 2021 5,639 0 View

How to isolate plasmid DNA from mammalian cells (HEK 293T) after transfection?

I have transfected the HEK 293T cells with plasmid having fluorescent protein expression. After sorting the positive cells (cells having fluorescent protein expression), i want to isolate the...

19 September 2019 1,891 3 View