Mohd Ahmad

7 Questions 14 Answers 0 Followers

Questions related from Mohd Ahmad

Are these HepG2 cells in any kind of stress?

I have recently started working with the HepG2 cell line. I am growing them in a High Glucose medium, with L-Glutamine and without phenol red. As I have seen many pictures of the HepG2 cells on...

13 November 2023 4,420 4 View

Is there any hightroughput method to measure the protein expression levels of 100's of protein variants simultaneously when transfected in human cell?

Hi, I have a synonymous variant library of a protein, and it has hundreds of variants. They are cloned in the Flp-In T-REx expression vector to work with the Flp-In system. We have worked with...

23 July 2023 3,974 1 View

Under CMV promoter, how strongly a heterologous gene is expressed? What is heterologous gene's mRNA % compare to total RNA & total mRNA in a cell?

I want to know that, when a heterologous gene is expressed under CMV promoter in mammalian cells, what is the percentage of this heterologous gene's mRNA in comparison to total cellular RNA and...

19 April 2023 5,966 2 View

Any optimized protocol for nucleofection of EBV-transformed lymphoblastoid B-cell lines (LCLs) with CRISPR vectors (12kb)?

I am trying to knock down a gene in EBV-transformed lymphoblastoid B-cell lines (LCLs) using the CRISPR system. For this purpose, first I tried the lipofectamine mediated transfection of cells but...

13 October 2021 5,651 0 View

How to label the HA tagged protein with anti-HA fluorescent antibody in Endoplasmic reticulum of Saccharomyces cerevisiae?

I have localised a protein in Endoplasmic reticulum of Saccharomyces cerevisiae. I have tagged this protein with HA tag. Now i want to label this protein with anti-HA fluorescent antibody for FACS...

23 December 2019 5,841 2 View

How to isolate plasmid DNA from mammalian cells (HEK 293T) after transfection?

I have transfected the HEK 293T cells with plasmid having fluorescent protein expression. After sorting the positive cells (cells having fluorescent protein expression), i want to isolate the...

19 September 2019 1,913 3 View

Is there any protocol for transforming Saccharomyces cerevisiae that is sensitive to the LiAc?

I have Saccharomyces cerevisiae strains that are deficient in some plasma membrane proteins that makes them sensitive to any stress and in the same way to Lithium acetate too. While using the...

14 September 2019 733 4 View