In ChIP-Seq it is usual to control for cromatin accesibility by using a so called "input" DNA. Is this also necessary/advisable/pointless to use a similar control for MeDIP-Seq?
best
Hi,
if you can exclude genomic aberrations in your samples (deletion, amplification) it may work without an input sample.
However, I would recommend to control for enrichment bias by using an in-vitro methylated DNA.
Good luck
Hi Norman, thanks for your reply
Could you please elaborate a bit more on the enrichment bias and teh in-vitro methylated DN?
santos
I've come to teh conclussion that an input DNA is important whenI havone compares for instance tumor vs normal tissue (or cells). Buthat it is really no us doenst really cointroibute much when oine compares for instance cellstheeated and untreatedone contribute with some compuncompund,, cultur ... Is that correct?the one fromteh same individuals
I apologize for the lot typos in my previous message. Something weird happened.
The message was intended to be as follows:
I've come to the conclusion that an input DNA is important when one compares for instance tumor vs normal tissue (or cells) from the same individuals. But that it doesn’t really contribute much when one compares for instance (cultured) cells treated and untreated (with some compound)… Is that correct?
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