Have you ever used cytotoxic T-cells in a cytotoxicity assay (eg. Cr51-release or something equivalent) directly after thawing them?

I have aliquots of expanded cytotoxic T-cells that I would like to use directly in a cytoxicity assay after thawing the cells without re-stimulating them. So far I've tried thawing them, resting...

09 October 2017 8,700 3 View

ThermoFisher Mouse T-activator Dynabeads suddenly stopped working for me. Has anybody else had a similar problem?

Hi all, I had been using ThermoFisher's mouse T-activator kit (Catalog number: 11456D) to expand CD8 T-cells from mouse splenocytes. For around 2 years I had great results, got 200-fold expansion...

01 February 2017 1,430 2 View

My EL4 cells had a massive die-off. Any idea why?

I've been working with the mouse thymoma line, EL4, and have had a couple occasions in which the cells have suddenly and completely died off. Is it known if these cells have a senescence limit...

31 December 2015 8,624 4 View

Which automated cell counter is best for handling small cells such as splenocytes and thymocytes (~8 micron diameter)?

We have an Invitrogen Countess counter which works really well for most mammalian cell lines. However, it seems to be less accurate when enumerating small cells - especially with respect to their...

31 December 2015 8,830 7 View

Does anybody know the proportion of Ova-specific OT-I T-cells from the OT-I mouse express a second TCR from rearrangement of the endogenous locus?

The natural TCR locus of this TCR transgenic mouse is still intact and is capable of rearranging. Is there any data out there regarding the frequency at which other productive TCRs are...

05 June 2015 4,323 2 View

Is the compensation value set in a flow cytometry experiment dependent on the brightness of the fluorophore?

If I've got a compensation control that is much brighter than my experimental sample, is the compensation value derived from this control still valid? Intuitively, I feel that more fluorescence...

10 November 2014 1,261 5 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

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03 March 2021 5,360 2 View

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02 March 2021 3,060 3 View

How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

What SPSS analysis should I run with one IV (with two levels) and two DVs?

Hi, I am trying to figure out which test would be most appropriate to run for the following question: Is there a difference in task blood pressure between smokers and non-smokers? There are two...

01 March 2021 8,235 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Does anyone know an alternative product for cardiac Troponin T antibody for Immunofluorescence (Thermo # MA512960)?

We have been using Thermo Fisher anti-cardiac Troponin T antibody for immunofluorescence staining in FFPE mouse heart sections but it was recently discontinued. The literature shows it to be the...

01 March 2021 4,937 1 View

How to simultaneously perform multiple biochemical assays?

I am a research scholar working on heavy metal stress on plants. I will be doing biochemical characterisation( protein, carbohydrate, proline, antioxidant enzymes and many more assays) at interval...

01 March 2021 6,999 3 View