Hello. To use in enzymatic hydrolysis which surfactant is better? I have read papers which use Tween 20 and Tween 80, but I do not know what is the difference in their application in enzymatic hydrolysis.
Polysorbate 20 (Polyoxyethylene sorbitan monolaurate) and Polysorbate 80 (Polyoxyetheylene sorbitan monooleate). Both are nonionic surfactants that are used widely as emulsifying agents in the preparation of pharmaceutical emulsions. Also, as solubilizing agents for a variety of substances, and as wetting agents in the formulation of oral/parentral suspensions. The difference between both polysorbates are in their physicochemical properties; i.e. HLB value, Hydroxyl vaue, Saponification value and Surface Tension,
Polysorbates enhance the hydrolytic activity and increased the enzymatic hydrolysis rate of the substrate, depending on the concentration and type of polysorbate. Both polysorbates have shown a positive effect on enzymatic hydrolysis. You can use whatever you want. But for cellulose and cellulose derivatives, I suggest you "Tween 80"
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