Is there a way to convert MPN (most probable number) to CFU (colony forming unit)?

I am conducting a meta-analysis and most of my studies report values for bacterial analysis in CFU. However, I have one study which reports its microbial values in MPN. What should I do?

29 April 2020 662 5 View

How to clone 3 pieces?

Hello, I have to clone 3 pieces together: 1. 2 Pcr made- the inserts. One 4200bp (13 ng/microliter), one 460bp(21ng/microliter) 2. Vector 3800bp(13ngng/mictoliter). All pieces were cut with...

27 April 2020 809 3 View

Subgroup analysis in small numbers allowed?

Hello, I had a question regarding subgroup analysis in small patient numbers. I am currently writing a paper on very rare tumours and the response to therapy in a real-world cohort, so no...

18 March 2020 4,983 16 View

Trying to produce lentivirus containing human-FasL in 293FT cells, but the cells keep dying after transfection. Anyone succeeded making FasL-Viruses?

I have a Lenti-FasL plasmid (CMV promoter) I bought from GeneCopoeia, and I am trying to produce Lentiviral particles containing the human FasL plasmid. For that I am using 293FT cells with...

24 July 2018 1,361 3 View

Since G6P dehydrogenase is a dimer, are 2 H+ ions released upon catalysis of G6P, not 1 as shown in the equation? Are they released as gas or ions?

I'm specifically interested in the reaction as it occurs in EMIT assays. Does H+ stay in solution, hence the need for precise buffering, or is it released as gas?

10 April 2018 4,123 6 View

Have you used the ProteinSimple Simple Western, and found that your molecular weights were different versus regular blot? How did you resolve this?

I am investigating the ProteinSimple Simple Western system, but have found that my proteins (and some ladders that I've used in the past) come out at very different molecular weights compared to...

10 November 2017 8,075 3 View

I am looking for a good resource for risk communication in public health. Can anyone make a recommendation?

05 June 2017 9,314 11 View

Bradford assay for exosomes?

11 July 2016 8,057 8 View

Air Quality/ Odor emergency response and management software?

Hi, I am looking for software to help regulators manage emergency response teams in case of extreme events of air pollution release or odor complaints from citizens. The software should: be...

27 September 2014 9,025 3 View

How to analyze a particular region of my simulation box in GROMACS?

Hi, I am using GROMACS 5.1.4 to simulate water in contact with a surface. I am only interested to calculate some properties of water near the surface. Let's say at a distance of x nm from the...

03 March 2021 9,989 2 View

How to get onecolumn document from standard IEEE ACCESS LateX template?

I have prepared a manuscript in IEEE Latex template, but for some purpose, I need to prepare a word file as well (detailed format is not required). Compared to two column .tex file, one column...

02 March 2021 1,830 3 View

How can the modification of garden soil cause global warming?

Hello, I am working on a soil project and I found a primary research article that tests how the modification of agricultural soil can cause global warming, using a thermal collector, however I...

02 March 2021 7,161 3 View

In Molecular Dynamics Simulation, how do I make changes to the force field according to my protein?

Hi In Molecular Dynamics Simulation, how do I make changes to the force field according to my protein? I got this error in the add-ion stage: Fatal error "./ amber99sb-ildn-HHH.ff / force...

01 March 2021 8,747 4 View

Does anyone know how to plot my station to a section plot on Ocean Data View (ODV)?

I made a vertical section plot with WOA .nc file on ODV, and now I want to plot my stations (csv. file) in it. Does anyone know how to import my points to the section? They are two different...

01 March 2021 3,610 1 View

Does anyone know to clarify these changes?

01 March 2021 1,290 2 View

How can I calculate the rdf of water molecules around 'Na' ions that are closest(adsorbed) to the nanoparticle(solute)?

Hi, I want to calculate the radial distribution function for water molecules around the Na+ ions adsorbed on the surface of a spherical nanoparticle using the gromacs trajectory dynamically? For...

28 February 2021 5,650 1 View

How can I convert .vesta file to a .cif file?

I've used the "export data" option in Vesta software to convert my unit cell into a cif file however when I use that cif in atomsk it shows error such as "can't read the position of atom 0". If I...

28 February 2021 4,966 3 View

What is Flat Lens Antenna?

28 February 2021 5,433 3 View

How can I assign a velocity along one direction to CO2 molecules in a molecular dynamics simulation using GROMACS?

Dear all, I am trying to perform a MD simulation about the interaction between CO2 and a polymer. I create my model, solvate it and perform the NVT and NPT equilibration. Now I am not able to...

26 February 2021 10,014 3 View