I prepared the histological tissue according to the recipe. There were no problems during cutting, but after the drying by 37°C I recognized that the tissue does not adhere to the slides. I can blow it away. And during a normal HE staining all slices peel away and I got no output. I totally do not know what was going wrong.
Hello Corinna,
What protocol exactly do you follow and with what biological material? I believe this information is essential for discussing the problem.
As to my personal experience: I had a similar problem too when cutting mouse hippocampal slices (fixated in PFA, cryoprotected in 30 % sucrose in PBS until they'd sink,embedded in OCT, cryosectioned into 30 µm sections, and picked up on Thermo Superfrost Plus slides to do cresyl violet staining). The problem has been solved completely by using subbed (aka gelatine-coated slides) which I prepare myself: no sample has floated away from since then. It is rather easy to prepare them, and there are many protocols for that on the Internet; I can share the one I use successfully if you want me to.
Best,
Max
Hi Corinna,
As Maksims said exact protocol's steps are necessary. However,in My experience, there are two main causes for the problem:
1- Time and temperature of tissue float (high temperature and over maintaining of tissue on water may be cause of the problem)
2- Commercial coated Slide! sometime self prepared slides provide better results than famous brands charged slides.
Good luck,
Hello , Carina
If you made frozen section from fornmalin or another fixative solution fixed tissues it will be. But if you use parafin embebed tissues please send (as previous dear collegues wrote) you protocol of tissue preparation and stain.
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