What happens to transcytotic vesicles in MDCKII cells growing on plastic dishes?

Hello everyone, I am doing some studies to compare antibodies in their recycling behaviour in MDCKII cells after receptor mediated internalization. For the purpose of simplifications, I am growing...

21 October 2017 2,779 1 View

Experience with pFRT/lacZeo vs pFRT/lacZeo2 ?

We want to create a RPE1 FlpIn cell line. For this we have to integrate either pFRT/lacZeo or pFRT/lacZeo2. The latter has a truncated SV40 promotor, so that you only get Zeo resistance if the...

21 December 2016 3,605 1 View

No title

07 December 2016 3,507 3 View

Does anybody know about research on psychopathy and drug dealing?

I am looking for research on psychopathy and drug dealing, does anybody have something on that subjetc. Thank you very much

30 March 2016 4,498 3 View

In how far can the bacterial kingdom be seen as a genetic continuum?

In other words, is it possible to build a genetic "vector space" where a bacteria can be identified by its "genetic coordinates", each coordinate being the homology to a "genetic reference" or...

07 February 2015 2,642 3 View

What is the best spatial resolution when isolated frozen-hydrated enzyme complexes are investigated in low-dose mode in tilt series? References?

Models of enzyme complexes not yet developed on the basis of other imaging techniques (e.g.X-ray) may be of interest in studies on enzyme function.

07 May 2014 9,807 2 View

Contextual approach to psychology research

Can somebody shed some clarity on how a contextual approach to qualitative research translates into a specific paradigm, epistemology, ontology and axiology in psychology? I understand that it is...

01 January 1970 6,946 3 View

How to code open-ended questions in SPSS?

Hello all, In SPSS I am going to code 2 open-ended questions. I have already read all the answers and I made a list of the most important categories to which I can code the answers. This question...

02 March 2021 1,757 4 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

How to define and get results (stresses) for certain points of a model in abaqus, using python? When the size of the model changes?

I want to do a parameter study of a aixsymmetric cylinder in Abaqus. I want to create several jobs where I get the stresses for 3 different points in my model. These points are defined. I wrote a...

01 March 2021 10,078 1 View

Which is suitable for use with Python? MySQL or SQL Server?

Which is suitable for use with Python? MySQL or SQL Server? What is your suggestion?

01 March 2021 3,422 3 View

How to eliminate Steady state error from Reinforcement learning genetic algorithm code?

The following code (see 1st 2 images attached) is used to produce PID controller values that are designed to control the system (G). The code finds the PID controller values (noted as k) by using...

28 February 2021 6,560 14 View

Can anyone provide the Support vector regression code structure of LIBSVM MATLAB or python ?

Please, if Gaussian Parameter optimization is possible in this code structure

28 February 2021 278 3 View

How to modify Johnson cook equation in ABAQUS?

I wanted to add an extra parameter to the existing Johnson cook equation in the ABAQUS simulation. How to add those extra parameters. Ref: A modified Johnson-Cook material model with strain...

28 February 2021 4,841 3 View

How can we simulate an hyperelastic-viscoelastic behavor with Ansys APDL?

I am working on modeling and simulation of biomecanical material behaviour, I have succeded on simulating skin using anisotropic hyperelastic material, on APDL and in our lab's finite element...

28 February 2021 552 3 View

No module named 'tensorflow.keras.backend.tensorflow_backend' ?

System Description Cuda 11.2 GeForce GTX 1080ti tensorflow version 2.4.1 keras version 2.2.0 python version 3.8 I was getting the below error when I try to run my code on the...

25 February 2021 6,673 2 View

How could I process data in RCT to include it in meta-analysis?

I'm in the process of doing a meta-analysis and have encountered some problems with the RCT data. One of my outcom is muscle strength. In one study, I have three different measurements of muscle...

25 February 2021 7,603 3 View