Hello everyone,
I am doing some studies to compare antibodies in their recycling behaviour in MDCKII cells after receptor mediated internalization. For the purpose of simplifications, I am growing the cells on plastic dishes instead of trans-membranes. Now I am wondering what happens to antibodies that would undergo the transcytose pathway? Do you know if I will detect it in the supernatant as it will be recycled ? Or will they be trapped in the transcytotic vesicles within the cells? I have not found informations of MDCKII cells growing on plastic dishes.
Thank you for your opinion!
There is no guarantee either the antibodies are recycled or not absorbed and two things to be considered,
1. you have to check first if the cells are healthy i mean sufficiently grown
2. to confirm whether the antibodies are internalized or not you can perform intracellular staining and flow cytometry
good luck
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