Dear fellow researchers,

does anyone know how the large Protein (469 kD) DNA PKcs gets transported into the nucleues ? I know that it exists in the nucleus "in abundance" and most of its function is studied in regard to DNA Repair which of course happens in the nucleus. However, it must be produced in the cytoplasm (right?) and therefore it somehow has to be transported into the nucleus. I read that this happens trough a nuclear localization sequence and importin which then interacts with the nuclear pore complex but I am not sure how this is possible for this extremely large protein and if there is any data at all ?

I can imagine that it gets threaded trhough in an elongated somewhat unfolded state is there evidence for this with other proteins ?

I thank you all for your help in advance.

- Benjamin