Have someone successfully used the ER alpha (Santa Cruz sc-8002) antibody in immunostaining ?

Santa Cruz now recommends this new antibody (F-10) but I can't find a publication using this reference. I'm working on human et non human primate hypothalamus and so far, it doesn't work. Is...

02 March 2021 10,003 2 View

How do I change a preprint to published?

I want to know how to change the status of an article.

21 February 2021 1,536 2 View

How to use Remote sensing and GIS applications for mapping the available medicinal plants within the locality/municipality?

Hello, I'm very interested in mapping and managing the conservation of the Philippine traditional medicinal plants, in which I think is very helpful in the field of medicine for further analysis...

21 February 2021 374 5 View

How can you adjust pH of SM buffer?

Good afternoon, please how can you adjust pH of phage SM buffer? I presume that main ingredient that has influence of pH is Tris/HCl buffer, but according to literature its range of pH is from 7...

27 January 2021 5,767 3 View

Where can I find plants pyr-GC-MS data?

25 January 2021 5,403 2 View

What can be the Research Design if I am looking for Correlation Coefficient and Regression Coefficient?

In our research our title includes the impact of academic anxiety on academic performance. This one is a quantitative research. Our data is numeric. Can someone suggest what can be the research...

21 January 2021 1,650 3 View

What would cause Cell Titre Glo assay wells to luminesce less than the media only wells?

Hi all, I'm doing a bunch of Cell Titre Glo assays to assess the effects of several inhibitors on 2 cell lines. Problem is, for some of the wells they seem to be luminescing less than the...

30 October 2020 1,513 2 View

Can someone give me an example for a questionnaire regarding roger's innovations in organizations?

Hello, I am looking for a questionnaire based on Roger's Diffusion of Innovations - Innovations in Organizations theory. Can someone give me an example?

15 September 2020 8,351 4 View

Are there studies on fallback fruits of avian frugivores?

Hello everybody! Fallback fruits are fruits of low nutritional value that in periods of fruit scarcity become the filler or staple of a frugivorous animal. Fallbacks seem to be well-studied in...

17 August 2020 7,923 4 View

Can I get some help and reading these results? I ran a regression analysis of students pre and post test grades?

After conducting a linear regression analysis of Interpretive Reading (Pre-test) scores (Table 12), the coefficient or Y-intercept associated with the pre-test scores, (0.696985136) is zero, and...

02 July 2020 7,309 1 View

How to grow E.coli on the low salt LB agar plate with 50 or 100ug/ml of Blasticidin?

Hello, I would like to ask how to grow E.coli which resists 50 or 100ug/ml of Blasticidin. I have tried many methods by using low salt Luria-Bertain(LB) agar plate. However, no colonies grow. Let...

01 March 2021 2,027 2 View

Can bacterial suspension be pipetted directly onto petri dish and agar poured onto it?

Hi, recently I encountered a protocol for plating which was not the usual method I have been practicing (pour LB agar onto plate, allow it to solidify, pipette bacterial suspension onto plate,...

28 February 2021 7,292 3 View

Difficulty in cloning of 1kb gene into vector?

I am facing difficulties in cloning a 1kb gene into a vector (pJIT163). I have my gene on interest (GOI) in pUC57 and want to clone in pJIT163 using SalI and BamHI restriction sites. I am getting...

25 February 2021 3,221 7 View

Can 5-FOA Diffuse Through Agar?

I am preparing to do a gene knockout in the filamentous fungi Coccidioides posadasii targeting pyrG/URA3 so that 5-FOA can be used for selection pressure instead of traditional antibiotic...

16 February 2021 169 4 View

How do I change g/l into mM for bacto agar. I am trying to get the millimolar content of sodium and chloride percentage in bacto agar. How do I do it?

I am doing a salt tolerance-related experiment and for that I need to relate my salt levels to that in bacto agar. I don't want my salt concentrations to increase due to already salt content...

09 February 2021 4,158 1 View

Transformation into the Stbl3 chemo competent cells?

03 February 2021 1,111 5 View

How to Remove DNA From a Sample Without Rupturing Yeast?

I transformed a plasmid into yeast and selected for transformants by growing them in a selective media (liquid, not agar plate). My experiment requires that I continually grow/ split these...

01 February 2021 9,936 2 View

What is the composition of the solid and broth agar to grow Streptomyces from freezed sample? what are extraction steps of products from broth agar?

Please what are the composition of the solid agar could I use to grow the bacteria? what are the composition of the broth agar could I use to grow the bacteria and how to extract the natural...

26 January 2021 5,006 1 View

What is the best way and technique for serial dilution of bactria to get accurate number of colony ?

Hello I am infecting cells with bacteria in a well and then I am doing serial dilution of this well 1 in 10 and culture the two last dilution on MHA agar I have difficulty in getting accurate...

26 January 2021 9,256 5 View

How to reduce or increase CFU/ml value ?

I collected the water sample from pond and 1 ml of pond sample diluted with 9ml with water and 100 microliter was cultured into the MacConkey agar plates .Finally,80 colonies were formed in the...

24 January 2021 1,925 3 View