Can I use Fluorescein Diacetate (FAD) to check the viability of my plant cells after the H2O2 treatment?

Hi all, I am treating my 8 day old Arabidopsis thaliana saplings with different concentrations of H2O2 for variable time periods. After the treatment I want to check the viability of cells...

10 November 2019 2,346 2 View

Is it required to quantify cDNA concentration before going for semi-quantitative PCR of any particular gene?

To prepare cDNA, I took the equal concentrations of RNA of both control and experiment samples. The volume of control and experiment samples during cDNA preparation was kept equal. Do I need to...

02 March 2017 9,756 6 View

How long overhang I can keep in my primer?

31 December 2016 153 7 View

Do I need to go for Dpn1 treatment?

07 August 2016 409 4 View

Why I'm getting extra protein bands with my specific protein band during Ni-NTA His-tagged protein purification?

I have expressed a protein of C. jejuni in E.coli M15 cells using pQE30 vector. My protein carries a N-terminal hexa his tag. I have purified my recombinant protein using Ni-NTA columns and I have...

07 August 2016 9,231 8 View

Transformation of E.coli, T7 expression strain with an expression vector having T5 promoter will work??

I have an expression vector carrying my gene of interest with T5 promoter and I want to transform it into BL21DE3 strain which is a T7 expression system. Just I want to know if doing so will not...

05 June 2016 2,074 3 View

How can I grow two different cell lines in one plate and even they don't merge in each other?

11 December 2015 2,725 4 View

What is the accuracy of epitope prediction software's?

I have no idea how these epitope prediction software's do work but I think using them will be somehow beneficial rather than designing primers blindly for the peptide synthesis. Before I use any...

08 September 2015 4,037 1 View

Why does my plasmid get stuck in the agarose gel well?

Hi, I've cloned my GOI into TA cloning vector( pDrive) vector, after I did transformation into DH5 alpha I got good number of colonies.After this when I'm doing plasmid isolation using...

07 August 2015 4,458 7 View

Is there any way to count cell number using phase contrast image? Can I calculate percentage differentiation using phase contrast image?

I'm having phase contrast image data of my previous cell culture where I have to count number of differentiated Mesenchymal Stem cells among normal cell population. can anyone please suggest me...

02 March 2015 6,342 8 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

How do you write a simplified C block code of this average-current control on PSIM?

This is a Buck (step-down) Converter and I am new to this program. I need to program with simplified C block and I actually don't know what to do. Can anyone show me how to do this? Or at least...

02 March 2021 8,233 1 View

Do you know good and user-friendly programs to measure plant quantitative traits?

For my research i will need to measure plant quantitative traits (especially leaves area and roots length, but would be nice to add some more information). I recently discovered...

01 March 2021 5,035 2 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Runs of nucleotides in Sanger sequencing?

I performed site directed mutagenesis, transformation, and then I sent out plasmids for Sanger sequencing and found out that there is extension of DNA just before the stop codon. I am not sure...

27 February 2021 547 3 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

Errors in DNA sequences?

I am trying to better understand the scope of DNA replication and sequencing errors, e.g. 1. I have seen similar error rates of 10e4 to 10e5 for cell & instrumental DNA replication,...

24 February 2021 4,397 3 View

Is it possible to set a breakpoint at a specific DYNAMIC instruction using the GNU Debugger (GDB)?

Using GDB, it is straightforward to debug and monitor a target program by setting a break-point at a specific instruction, since the instruction addresses are known. However, we have two...

24 February 2021 1,598 2 View

Implementation of chance-constrained optimization problem via scenario approach?

23 February 2021 7,826 2 View

I am looking for a simple software program to model 3D chemical bonds. The purpose is to generate high-quality graphical abstracts. Any suggestion?

Hello everyone: I am looking for a simple software program to model chemical bonds. The purpose is to generate a high-quality graphical abstract for a top leading journal in the field of...

23 February 2021 8,012 3 View