13 Questions 17 Answers 0 Followers
Questions related from Avinash Sharma
Hi all, I am treating my 8 day old Arabidopsis thaliana saplings with different concentrations of H2O2 for variable time periods. After the treatment I want to check the viability of cells...
11 November 2019 2,503 2 View
To prepare cDNA, I took the equal concentrations of RNA of both control and experiment samples. The volume of control and experiment samples during cDNA preparation was kept equal. Do I need to...
03 March 2017 9,830 6 View
I want to design a primer where I want to insert an overhang of size 32bp whereas the size of annealing sequence is 32bp( GC=56%). Please tell me your views.
01 January 2017 285 7 View
I performed a PCR mediated deletion of my vector which was carrying an unwanted gene (Chey 183 bp). I designed two primers with desired restriction sites in order to clone any gene in place of...
08 August 2016 493 4 View
I have expressed a protein of C. jejuni in E.coli M15 cells using pQE30 vector. My protein carries a N-terminal hexa his tag. I have purified my recombinant protein using Ni-NTA columns and I have...
08 August 2016 9,296 8 View
I have an expression vector carrying my gene of interest with T5 promoter and I want to transform it into BL21DE3 strain which is a T7 expression system. Just I want to know if doing so will not...
06 June 2016 2,166 3 View
I want to study in-vitro host-pathogen interaction and subsequent immune response. In following experiment I would like to have two different cell lines in one plate say epithelial cell line and...
12 December 2015 2,790 4 View
I have no idea how these epitope prediction software's do work but I think using them will be somehow beneficial rather than designing primers blindly for the peptide synthesis. Before I use any...
09 September 2015 4,091 1 View
Hi, I've cloned my GOI into TA cloning vector( pDrive) vector, after I did transformation into DH5 alpha I got good number of colonies.After this when I'm doing plasmid isolation using...
08 August 2015 4,588 7 View
I want to know the name of any tool or program which I can download easily.I want to align two sequences side by side and and want to see the base pairs which are matching and the gaps. Also it...
05 May 2015 9,397 3 View
I'm having phase contrast image data of my previous cell culture where I have to count number of differentiated Mesenchymal Stem cells among normal cell population. can anyone please suggest me...
03 March 2015 6,395 8 View
I have been trying bFGF and Forskolin protocol to differentiate Mesenchymal Stem Cells into neuronal lineage cells using a 35 mm dish but I found my cells get overcrowded within the duration of...
12 December 2014 8,991 7 View
I have purified a protein of bacterial origin which is known to be a fibronectin binding protein. I want to confirm the biological activity of the protein I am having, I have also got the human...
01 January 1970 5,398 1 View
