Hello
I was performing the semi-dry transfer of my gel to the membrane for western blot What is the transfer buffer recipe that you guys use for semi-dry transfer? I saw a different recipe for wet mode and semi-dry transfer mode on Abcam website?
I have not tried the dry method (since I don’t have an iBlot/iFlex) or the semi-dry method, but the wet method transfer buffer I’ve used is:
800 ml ddH2O
100 ml 10x Tris-Glycine, and
100 ml methanol
Hi Vikrant,
I generally use Towbin buffer with SDS for semi-dry transfer. The buffer composition is given below. I use 0.1% SDS and this has worked well for me. It might be a good idea to look at the buffer recommendation of the company that has manufactured the transfer apparatus - I know that some of the instruction manuals of these machines mention the buffer composition.
25 mM Tris
192 mM glycine
20% (v/v) methanol
0.025–0.1% SDS
Make up with ddH2O
Good luck with you transfer!
Bhavana Kayyar Thanks for your response. This is the exact recipe that I am using for the transfer. I was just curious if someone had tried some different recipe for semi-dry transfer.
- Badges
- Science topic
- Similar topics
- Gels