Brandon Veltri

2 Questions 15 Answers 0 Followers

Questions related from Brandon Veltri

Do you have to clean out the his resin from a column if you are doing successive protein purifications?

I’m still a little new to this technique [of purifying his-tagged proteins]. I know there are protocols for recharging the columns but does the resin need to be removed before purifying the next...

12 December 2019 6,071 7 View

Can 1.1 buffer (NEB) be used with a digest using Kpn1 and BamH1?

I am trying to digest a pET-11a SUMO vector with Kpn1 and BamH1 (expected weights of both bands approximately 5770 bp and 210 bp, respectively), but I am getting mixed information on the NEB...

06 June 2019 8,631 2 View