Vikrant Mehta

10 Questions 47 Answers 0 Followers

Questions related from Vikrant Mehta

Semi Dry vs Wet Transfer recipe?

Hi I found that there are two different recipes for transfer depending on the mode (Semi-dry or wet) of the transfer. Why is it so? If anybody has prior experience with the use of both the...

12 December 2019 3,966 3 View

Transfer buffer recipe?

Hello I was performing the semi-dry transfer of my gel to the membrane for western blot What is the transfer buffer recipe that you guys use for semi-dry transfer? I saw a different recipe for...

11 November 2019 8,635 3 View

Duration of transfection to induce p53 silencing?

Hi All How long does it take to silence the p53 gene in breast cancer cells? Does the WT or mutant p53 status affect the transfection efficiency? Any other inforrmation regarding transfection...

07 July 2019 831 3 View

Molecular weight of protein is low but signal is at higher molecualr weight ?

The mol wt of my protein of interest is about 27 Kda but i am getting the signal at approx 50Kda. I am Using polyclonal antibody and the standard 12% gel for the detection. Running gel conditions...

06 June 2019 3,837 6 View

Cells found contaminated upon thawing?

Hi I froze the HEK 293 cells some 3 months ago in the Synth-a-freeze medium. When i started thawing them back they are heavily contaminated with bacterial growth. i have lost 4 aliquotes now due...

03 March 2019 8,346 1 View

Ponceau S Stain prior to probing the membrane with antibody?

Hi everyone If i stain my NC membrane with Ponceau Stain prior to probing with antibody of interest, would it interfere with the antibody binding and the subsequent development of the membrane for...

10 October 2018 594 5 View

HBL-100 Normal or Normal like or transformed?

Hi I am confused regarding the status of HBL 100. Some mention it as 'normal' cell line while others state that it is 'normal like' and another is it is 'transformed'. A clear and precise...

08 August 2018 2,771 3 View

Cells adhering at the edges of the culture flask/dishes?

Hi I have noticed one thing in cell culture is that the cells love to attach themselves at the side of the the culture vessels more than at any other part of the flask. Also, the these cells...

08 August 2018 6,245 5 View

RIPA lysis buffer?

Hi everyone I made a fresh RIPA buffer using foll. composition 1) Tris Buffer 50mM (7.5 pH) 2)150mM NaCl 3)1%NP-40 4)0.5% Sod. deoxycholate 5)Protease inhibitor After lysate was run on SDS-PAGE...

06 June 2018 422 5 View

Is there any dedicated site or a research article where i can find the status of p53 (WT or mutated) in various cell lines?

I need to know the p53 status of HSC-T6 and HK-2 cell lines. Can anyone help?

04 April 2018 555 3 View