Sagar Rayamajhi

6 Questions 10 Answers 0 Followers

Questions related from Sagar Rayamajhi

Tracking fluorescently labelled extracellular vesicles in tissue?

I have tissue treated with fluorescently labeled EVs. What is the best way to process the tissue for sectioning that can retain the fluorescence? I have tried cryostat sectioning. But the tissue...

18 January 2022 3,988 1 View

Solubility of Fe(III)-NOTA macrocyclic complex?

I prepared Fe(III)-NOTA complex. NOTA ( 2,2′,2”-(1,4,7-triazacyclononane-1,4,7-triyl)triacetic acid , Chematech) in acetic acid and FeCl3.6H20 in water was used. The resulting yellow colored...

19 December 2019 5,528 1 View

What type of 96 well plate is best for reading absorbance data?

Do microplate materials and their different kinds(round bottom, flat bottom) affect the absorbance reading of analyte? We have Epoch gen5 version3 microplate reader for reading absorbance data of...

16 November 2016 1,559 8 View

For making standard Quercetin solution, normally 70-80% ethanol is used as solvent. Can we use absolute ethanol?

While making standard Quercetin solution for the determination of flavonoids, it is dissolved in 70-80% ethanol. Can we use absolute ethanol so that we can get maximum solubility of quercetin in...

30 August 2016 3,574 9 View

Can a colored compound not have a lambda max in Visible range of light?

I have a light red colored solution. But when analyzing its absorbance curve in visible region, there is no lambda max. Instrument Used: Biotek Epoch Multi-plate reader There is no problem with...

01 January 1970 465 11 View

Problem with lambda max shift with higher concentrated solution? Should we not consider absorbance value greater than 1?

We had a blue colored solution whose lambda max should be around 750 nm. At low concentrated solution (100ug/ml), absorbance value upto 0.7, the lambda max was observed around 740 nm. But in the...

01 January 1970 8,962 2 View