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Questions related from Sagar Rayamajhi
I wanted to show membrane protein topology in 3-dimensional form with membrane intact. I wanted to show extracellular region, membrane region and intracellular region of protein in 3-d form. I...
19 June 2024 1,087 1 View
Is there a commercially available antibody that specifically targets the surface-tethered carboxy-terminal domain of MUC16 and not the cleaved extracellular domain of MUC16 (CA125)? Thank...
14 August 2023 1,804 0 View
I have tissue treated with fluorescently labeled EVs. What is the best way to process the tissue for sectioning that can retain the fluorescence? I have tried cryostat sectioning. But the tissue...
18 January 2022 4,061 1 View
I prepared Fe(III)-NOTA complex. NOTA ( 2,2′,2”-(1,4,7-triazacyclononane-1,4,7-triyl)triacetic acid , Chematech) in acetic acid and FeCl3.6H20 in water was used. The resulting yellow colored...
19 December 2019 5,611 1 View
I want to calculate T1 relaxation time of Magnevist from phantom image. I have phantom images corresponding to the different dilution of magnevist and water at different repetition time (TR). I...
08 February 2019 8,221 3 View
I need free software (open source or that can be found in cracked version) and relatively simple one (that doesn't require coding) for doing PCA for medium sample size data (19). Sample size:...
22 February 2017 2,194 13 View
I need Uniform heating in paper/glass microfiber for carbohydrate and lipid assay. Microwave heating can provide relatively uniform heat. But does it interfere with Carbohydrates and lipids?
30 November 2016 5,168 1 View
Glass microfibre filter and Thin layer chromatography plate are made up of silica materials. Is there any significant differences between these two? It is for the research of developing paper...
23 November 2016 1,322 2 View
Do microplate materials and their different kinds(round bottom, flat bottom) affect the absorbance reading of analyte? We have Epoch gen5 version3 microplate reader for reading absorbance data of...
16 November 2016 1,644 8 View
In some literature, I have found 5% Phenol, while in some 80-90%. How much is best? Also, For making standard glucose solution for calibration curve, how much concentration range is effective?
14 September 2016 1,503 6 View
While making standard Quercetin solution for the determination of flavonoids, it is dissolved in 70-80% ethanol. Can we use absolute ethanol so that we can get maximum solubility of quercetin in...
30 August 2016 3,644 9 View
I have a light red colored solution. But when analyzing its absorbance curve in visible region, there is no lambda max. Instrument Used: Biotek Epoch Multi-plate reader There is no problem with...
01 January 1970 567 11 View
We had a blue colored solution whose lambda max should be around 750 nm. At low concentrated solution (100ug/ml), absorbance value upto 0.7, the lambda max was observed around 740 nm. But in the...
01 January 1970 9,067 2 View
