How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

What are the values needed for gabor filter's parameter for cifar10 data?

Hi, I implemented a code to gabor filter cifar10 data but the images after being filtered and stacked are not clear like the original images. I think the problem is in the way I am using the...

03 March 2021 6,317 1 View

Can we block unwanted images during the x-ray prediction?

i am try to classify the x-ray images. During classification , can i block unwanted images (except x-ray image).

03 March 2021 7,100 1 View

No title

03 March 2021 5,360 2 View

What techniques can I use to determine the miscibility of polymer blends?

The term miscibility refers to the single-phase state in thermodynamics. I do not mean the compatibility of different components. To determine the miscibility I know several techniques such as...

03 March 2021 4,107 4 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

The procedure compute?

02 March 2021 5,204 3 View

Can I use saponin to permeabilize cell to measure mitochondrial respiration?

Saponin and digitonin both can permeabilize cells. However, based on many respiration assay protocols I read, saponin is used for tissue permeabilization and digitonin is used for cell...

11 January 2021 2,151 3 View

What is the chemical composition of Cucumis melo roots?

I have found an old pharmaceutical research from 1833 in which emetic power of Cucumis melo roots was confirmed. I cannot find data about chemical constituents of the Cucumis melo roots. Their...

30 October 2020 3,633 3 View

Non-specific nuclear staining with immunofluorescence microscopy?

01 February 2020 7,039 1 View

Which factors could affect IFN-g staining with antibody?

Hello everyone I am trying to evaluate IFN-g secreting CD4+ T cells in healthy individuals by flow cytometry. The following protocol is what i use for stimulation and staining: 1. A total of 1...

01 February 2020 6,138 7 View

How to quantify total saponin content from plant extracts?

*aqueous extract

08 September 2019 5,614 5 View

How to quantify total saponin content from plant extracts?

*aqueous extract

05 September 2019 4,521 4 View

What is the best permeabilizing agent to detect soluble cytoplasmatic and nucleic proteins by flow cytometry?

I need to detect and measure the abundance of cytoplasmatic proteins (AIM2, GBP1, GBP4) and some nucleic proteins (IFI16)using direct detection by flow cytometry with fluorochrome-conjugated...

11 May 2019 9,102 2 View

What is the best Saponin Extraction technique?

Which method of extraction can yield maximum saponin extract from plant part?

04 May 2019 2,705 3 View

How we can reduce Foaming / Froth formation during Hydro-alcoholic extraction?

We are performing hydro - alcoholic extraction of saponin from leaves of Ivy leaf. during extraction heaving foam and froth formation. that makes the process difficult to monitor and filter.During...

04 May 2019 356 1 View

What is the best permeabilizing agent to detect soluble cytoplasmatic and nucleic proteins by flow cytometry?

I need to detect and measure the abundance of cytoplasmatic proteins (AIM2, GBP1, GBP4) and some nucleic proteins (IFI16)using direct detection by flow cytometry with fluorochrome-conjugated...

04 May 2019 7,582 2 View