16 Questions 8 Answers 0 Followers
Questions related from Nimashi Herath
This is regarding the Total phenolic content assay. Initially, I used distilled water to prepare the gallic acid standard curve. But because of dissolution problems in samples in Distilled water,...
20 November 2022 9,594 2 View
Let’s say I got the following results for GC-MS analysis: How could I choose the correct compound present in my sample?
01 January 1970 7,935 1 View
Does anyone have a descriptive standard protocol for total flavonoid content assay and for Total flavanol content assay with a reference?
01 January 1970 8,910 0 View
Does anyone have a standard protocol for determining the total saponin content with a reference?
01 January 1970 5,425 4 View
I'm new to the FRAP assay. When I mixed FeCl3 with TPTZ before adding buffer, I got a dark blue solution instead of a straw-brown solution. I used the following method: 2 ml of acetate buffer...
01 January 1970 463 0 View
Does anyone have a descriptive standard protocol for the total phenolic content assay with a reference?
01 January 1970 6,074 1 View
Do any of you here have any experience with Tyrosinase inhibitory activity? What are the chemicals needed for the assay and experimental procedure? What are the difficulties of this evaluation?
01 January 1970 1,337 2 View
Is there a relationship between the ABTS and DPPH assays? Let’s say I am performing an ABTS and a DPPH assay for 5 extracts, and I am going to compare them by giving rankings. Are both the DPPH...
01 January 1970 4,216 1 View
How should make 1 U/mL solution of alpha glycosidase using 100 UN enzyme?
01 January 1970 2,167 2 View
I'm considering using enzyme assay kits instead of the standard protocols for inhibiting amylase, lipase, acetylcholinesterase, and glycosidase. What are the advantages and disadvantages of...
01 January 1970 2,791 2 View
I am working on an alpha-glucosidase assay. But when I apply PNPG as the substrate, the color is not developing. Why is it happening? Note: I prepared a fresh solution of PNPG and prepared the...
01 January 1970 9,133 2 View
How do we prepare 333 U/ml enzyme from 7164 units/mg solid of Tyrosinase enzyme?
01 January 1970 5,195 1 View
Does anyone have a tyrosinase inhibitory assay protocol with calculations?
01 January 1970 1,161 0 View
I am currently seeking information about any upcoming online conferences in the field of multidisciplinary science that are scheduled to take place in the year 2023. If anyone has knowledge of...
01 January 1970 2,634 0 View
Do any of you here have any experience with angiotensin-converting enzyme (ACE) activity assay? What are the chemicals needed for the assay and experimental procedure? What are the difficulties...
01 January 1970 3,174 1 View
Has anyone here tried vacuum oven drying your plant sample instead of conventional drying? If so, what results did you get? What are the parameters you used for the drying?
01 January 1970 1,233 1 View
