Nimashi Herath

15 Questions 8 Answers 0 Followers

Questions related from Nimashi Herath

Total phenolic content assay?

This is regarding the Total phenolic content assay. Initially, I used distilled water to prepare the gallic acid standard curve. But because of dissolution problems in samples in Distilled water,...

20 November 2022 9,548 2 View

"What is the Best Approach for Identifying the Correct Compound in GC-MS Analysis Using Agilent MassHunter Software?"

Let’s say I got the following results for GC-MS analysis: How could I choose the correct compound present in my sample?

01 January 1970 7,855 1 View

Total flavonoid content assay and Total flavanol content assay

Does anyone have a descriptive standard protocol for total flavonoid content assay and for Total flavanol content assay with a reference?

01 January 1970 8,840 0 View

Total saponin content

Does anyone have a standard protocol for determining the total saponin content with a reference?

01 January 1970 5,377 4 View

Why does the Frap reagent turn blue while preparing it?

I'm new to the FRAP assay. When I mixed FeCl3 with TPTZ before adding buffer, I got a dark blue solution instead of a straw-brown solution. I used the following method: 2 ml of acetate buffer...

01 January 1970 393 0 View

Total phenolic content

Does anyone have a descriptive standard protocol for the total phenolic content assay with a reference?

01 January 1970 6,022 1 View

Tyrosinase inhibitory activity

Do any of you here have any experience with Tyrosinase inhibitory activity? What are the chemicals needed for the assay and experimental procedure? What are the difficulties of this evaluation?

01 January 1970 1,292 2 View

Is there a relationship between the ABTS and DPPH assays?

Is there a relationship between the ABTS and DPPH assays? Let’s say I am performing an ABTS and a DPPH assay for 5 extracts, and I am going to compare them by giving rankings. Are both the DPPH...

01 January 1970 4,175 1 View

Alpha glycosidase enzyme preparation

How should make 1 U/mL solution of alpha glycosidase using 100 UN enzyme?

01 January 1970 2,117 2 View

Enzyme assay kits

I'm considering using enzyme assay kits instead of the standard protocols for inhibiting amylase, lipase, acetylcholinesterase, and glycosidase. What are the advantages and disadvantages of...

01 January 1970 2,738 2 View

Facing problem in alpha glucosidase assay?

I am working on an alpha-glucosidase assay. But when I apply PNPG as the substrate, the color is not developing. Why is it happening? Note: I prepared a fresh solution of PNPG and prepared the...

01 January 1970 9,092 2 View

Tyrosinase assay- Working enzyme solution preparation

How do we prepare 333 U/ml enzyme from 7164 units/mg solid of Tyrosinase enzyme?

01 January 1970 5,146 1 View

Tyrosinase inhibitory assay protocol

Does anyone have a tyrosinase inhibitory assay protocol with calculations?

01 January 1970 1,116 0 View

Upcoming online conferences -2023

I am currently seeking information about any upcoming online conferences in the field of multidisciplinary science that are scheduled to take place in the year 2023. If anyone has knowledge of...

01 January 1970 2,581 0 View

Antihypertensive Activity Assay

Do any of you here have any experience with angiotensin-converting enzyme (ACE) activity assay? What are the chemicals needed for the assay and experimental procedure? What are the difficulties...

01 January 1970 3,130 1 View